MicroRNA-223 expression in neutrophils in the early phase of secondary damage after spinal cord injury

被引：70

作者：

Izumi, Bunichiro ^{[1
]}

Nakasa, Tomoyuki ^{[1
]}

Tanaka, Nobuhiro ^{[1
]}

Nakanishi, Kazuyoshi ^{[1
]}

Kamei, Naosuke ^{[1
]}

Yamamoto, Risako ^{[1
]}

Nakamae, Toshio ^{[1
]}

Ohta, Ryo ^{[1
]}

Fujioka, Yuki ^{[1
]}

Yamasaki, Keiichiro ^{[1
]}

Ochi, Mitsuo ^{[1
]}

机构：

[1] Hiroshima Univ, Dept Orthopaed Surg, Grad Sch Biomed Sci, Minami Ku, Hiroshima 7348551, Japan

来源：

NEUROSCIENCE LETTERS | 2011年 / 492卷 / 02期

关键词：

Spinal cord injury; MicroRNA; MicroRNA-223; Neutrophil; Inflammatory response; INFLAMMATORY RESPONSE; BRAIN;

D O I：

10.1016/j.neulet.2011.01.068

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

MicroRNA (miR)s are short non-coding RNAs that suppress the translation of target genes, and play an important role in gene regulation. Despite this prominence, there are few reports that refer to the expression of miRs after spinal cord injury (SCI). Previously, we reported on miR-223 expression after SCI in mice. The purpose of this study is to reveal the distribution of miR-223 and identify the cells that express miR-223 in the injured spinal cord. Quantitative polymerase chain reaction analysis revealed high expression of miR-223 at 12 h after SO. Double staining of in situ hybridization and immunohistochemistry showed that the signals of miR-223 merged with Gr-1 positive neutrophils. Our data indicate that miR-223 might regulate neutrophils in the early phase after SCI. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：114 / 118

页数：5

共 23 条

[1] A SENSITIVE AND RELIABLE LOCOMOTOR RATING-SCALE FOR OPEN-FIELD TESTING IN RATS [J].