Multiple essential roles for EzrA in cell division of Staphylococcus aureus

被引：75
作者：
Steele, Victoria R. [1 ]
Bottomley, Amy L. [1 ]
Garcia-Lara, Jorge [1 ]
Kasturiarachchi, Jagath [1 ]
Foster, Simon J. [1 ]
机构：
[1] Univ Sheffield, Krebs Inst, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England
来源：
MOLECULAR MICROBIOLOGY | 2011年 / 80卷 / 02期
基金：
英国生物技术与生命科学研究理事会;
关键词：
FTSZ-INTERACTING PROTEIN; BACILLUS-SUBTILIS; ESCHERICHIA-COLI; STREPTOCOCCUS-PNEUMONIAE; TRIMERIC COMPLEX; MEMBRANE-PROTEIN; GENE; IDENTIFICATION; RESISTANCE; BACTERIA;
D O I：
10.1111/j.1365-2958.2011.07591.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
P>In Bacillus subtilis, EzrA is involved in preventing aberrant formation of FtsZ rings and has also been implicated in the localization cycle of Pbp1. We have identified the orthologue of EzrA in Staphylococcus aureus to be essential for growth and cell division in this organism. Phenotypic analyses following titration of EzrA levels in S. aureus have shown that the protein is required for peptidoglycan synthesis as well as for assembly of the divisome at the midcell and cytokinesis. Protein interaction studies revealed that EzrA forms a complex with both the cytoplasmic components of the division machinery and those with periplasmic domains, suggesting that EzrA may be a scaffold molecule permitting the assembly of the division complex and forming an interface between the cytoplasmic cytoskeletal element FtsZ and the peptidoglycan biosynthetic apparatus active in the periplasm.
引用
收藏
页码：542 / 555
页数：14
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