A double signal amplification-based homogeneous electrochemical sensor built on catalytic hairpin assembly and bisferrocene markers

被引：5

作者：

Cao, Hai Yong ^{[1
]}

Lu, Bo ^{[1
]}

Cheng, Lin ^{[1
]}

Xu, Bin Xiang ^{[1
]}

Wang, Mei ^{[1
]}

Xu, Wei ^{[1
]}

Cui, Han Feng ^{[1
]}

机构：

[1] JiangXi Univ Tradit Chinese Med, Dept Pharm, Nanchang 330004, Jiangxi, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2021年 / 632卷

基金：

中国国家自然科学基金;

关键词：

Electrochemical sensor; Bisferrocene; Homogeneous solution; Hg2+detection; Catalytic hairpin self-assembly; PEROXIDASE-LIKE ACTIVITY; HG2+ DETECTION; GRAPHENE OXIDE; ORGANIC FRAMEWORK; MERCURY(II) IONS; QUANTUM DOTS; LABEL-FREE; BIOSENSOR; APTASENSOR; GOLD;

D O I：

10.1016/j.ab.2021.114140

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A facile, sensitive and unmodified Hg2+ homogeneous electrochemical sensor based on bisferrocene signal markers and catalytic hairpin self-assembly (CHA) was built on a gold disk electrode. Three hairpin probes were designed, in which thiol was labeled at both ends of the hairpin probe 1(HP1), while bisferrocene, a redox signal marker, was labeled at both ends of the hairpin probe 2(HP2) and hairpin probe 3(HP3). Due to the Hg2+ mediated thymine-Hg (II)-thymine (T-Hg2+-T) structure, when Hg2+ is introduced, the T-Hg2+-T that occurred between the probe DNA and helper DNA could open the hairpin structure of probe DNA and form a rigid DNA triangles structure by CHA. Simultaneously, four bisferrocene signal markers also reached the surface of the electrode and built potential-assisted Au-S self-assembly to achieve signal amplification. Under the optimized condition, the sensor can achieve good electrochemical response Hg2+detection, and the detection limit is as low as 0.6 pM. furthermore, this sensor has high selectivity for Hg2+ detection.

引用

页数：7