Development and application of estradiol sensor based on layer-by-layer assembling technique

被引：23

作者：

Hao, Gugu ^{[1
,2
]}

Zheng, Dongyun ^{[1
]}

Gan, Tian ^{[1
]}

Hu, Chengguo ^{[1
]}

Hu, Shengshui ^{[1
,2
]}

机构：

[1] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

[2] Chinese Acad Sci, State Key Lab Transducer Technol, Inst Elect, Beijing 100864, Peoples R China

来源：

JOURNAL OF EXPERIMENTAL NANOSCIENCE | 2011年 / 6卷 / 01期

关键词：

pencil graphite electrode; electrochemical sensor; layer-by-layer assembling; estradiol; GLASSY-CARBON ELECTRODE; PERFORMANCE LIQUID-CHROMATOGRAPHY; SENSITIVE DETERMINATION; HORSERADISH-PEROXIDASE; VOLTAMMETRIC DETECTION; PENCIL ELECTRODE; FILM; ESTROGENS; ACID; DNA;

D O I：

10.1080/17458081003752988

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple strategy for the modification of disposable combination graphite-clay electrode, pencil graphite electrode (PGE), by multi-walled carbon nanotube-gold nanoparticle (MWNT-GNP) composite multilayers was proposed as a sensor to determine estradiol. The modification was carried out by the layer-by-layer (LBL) assembling of polyethyleneimine (PEI), decorated GNPs and polyacrylic acid (PAA) functionalised MWNTs via electrostatic interactions on a negatively charged PGE. The resulting MWNT-GNP multilayer modified PGE (MWNT-GNP/PGE) exhibited a sensitive electrochemical response towards the oxidation of estradiol at 0.90 V. Under optimal working conditions, the oxidation current of estradiol at MWNT-GNP/PGE increased linearly with its concentration in the range 7.0 x 10-8-4.2 x 10-5 mol l-1, with a low-detection limit of 1.0 x 10-8 mol l-1 for 200 s accumulation at an open circuit. This sensor was successfully applied to the detection of estradiol in blood serums.

引用

页码：13 / 28

页数：16

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