CALCIUM-DEPENDENT PROTEIN KINASE38 regulates flowering time and common cutworm resistance in soybean

被引：30

作者：

Li, Xiao ^{[1
]}

Hu, Dezhou ^{[1
]}

Cai, Linyan ^{[1
]}

Wang, Huiqi ^{[1
]}

Liu, Xinyu ^{[1
]}

Du, Haiping ^{[2
]}

Yang, Zhongyi ^{[1
]}

Zhang, Huairen ^{[1
]}

Hu, Zhenbin ^{[3
]}

Huang, Fang ^{[1
]}

Kan, Guizhen ^{[1
]}

Kong, Fanjiang ^{[2
]}

Liu, Baohui ^{[2
]}

Yu, Deyue ^{[1
]}

Wang, Hui ^{[1
]}

机构：

[1] Nanjing Agr Univ, Natl Ctr Soybean Improvement, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

[2] Guangzhou Univ, Sch Life Sci, Guangzhou 510006, Peoples R China

[3] St Louis Univ, Dept Biol, St Louis, MO 63103 USA

来源：

PLANT PHYSIOLOGY | 2022年 / 190卷 / 01期

基金：

欧盟地平线“2020”; 中国国家自然科学基金;

关键词：

DEPENDENT PROTEIN-KINASE; ETHYLENE BIOSYNTHESIS; S-ADENOSYLMETHIONINE; ARABIDOPSIS; CPK28; DEFENSE; PHOSPHORYLATION; RICE; IDENTIFICATION; EXPRESSION;

D O I：

10.1093/plphys/kiac260

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A calcium-dependent protein kinase positively regulates flowering time and negatively regulates resistance to the common cutworm in soybean and was artificially selected during domestication. Photoperiod-sensitive plants such as soybean (Glycine max) often face threats from herbivorous insects throughout their whole growth period and especially during flowering; however, little is known about the relationship between plant flowering and insect resistance. Here, we used gene editing, multiple omics, genetic diversity and evolutionary analyses to confirm that the calcium-dependent protein kinase GmCDPK38 plays a dual role in coordinating flowering time regulation and insect resistance of soybean. Haplotype 2 (Hap2)-containing soybeans flowered later and were more resistant to the common cutworm (Spodoptera litura Fabricius) than those of Hap3. gmcdpk38 mutants with Hap3 knocked out exhibited similar flowering and resistance phenotypes as Hap2. Knocking out GmCDPK38 altered numerous flowering- and resistance-related phosphorylated proteins, genes, and metabolites. For example, the S-adenosylmethionine synthase GmSAMS1 was post-translationally upregulated in the gmcdpk38 mutants. GmCDPK38 has abundant genetic diversity in wild soybeans and was likely selected during soybean domestication. We found that Hap2 was mostly distributed at low latitudes and had a higher frequency in cultivars than in wild soybeans, while Hap3 was widely selected at high latitudes. Overall, our results elucidated that the two distinct traits (flowering time and insect resistance) are mediated by GmCDPK38.

引用

页码：480 / 499

页数：20

共 91 条

[1] FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex [J].