Effect of preservatives for food grade C-Phycoerythrin, isolated from marine cyanobacteria Pseudanabaena sp

被引:50
作者
Mishra, Sanjiv K. [1 ]
Shrivastav, Anupama [1 ]
Pancha, Imran [1 ]
Jain, Deepti [1 ]
Mishra, Sandhya [1 ]
机构
[1] CSIR, Cent Salt & Marine Chem Res Inst, Discipline Marine Biotechnol & Ecol, Bhavnagar 364021, Gujarat, India
关键词
Citric acid; C Phycoerythrin; Degradation; Preservative; Stability; PHYCOCYANIN; SPIRULINA; ANTIOXIDANT; STABILITY; PHYCOBILIPROTEINS; BIOTECHNOLOGY;
D O I
10.1016/j.ijbiomac.2010.08.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
C-Phycoerythrin is water soluble red color chromo-protein which is used as a natural food colorant The effect of selected edible preservatives like citric acid sodium chloride sucrose and calcium chloride on the stability of C-Phycoerythrin at 0 +/- 5 C and 35 +/- 5 C was studied in aqueous solution Experiment was carried out to select a stabilizing agent having Hofmeister series behavior acting on hydrophobic interactions The denaturation of phycoerythrin with urea as denaturant and effect of different pH on C-Phycoerythrin was studied Citric acid (4 mg/ml) was observed to be one of the best preservative for C-Phycoerythrin at 35 +/- 5 degrees C and 0 +/- 5 C in aqueous solution for 45 days Citric acid was able to maintain the stability of C-Phycoerythrin in the solution The amount of C-Phycoerythrin left in the solution containing citric acid after 30 and 45 days was 46 and 37 8% respectively at higher temperature (C) 2010 Elsevier B V All rights reserved
引用
收藏
页码:597 / 602
页数:6
相关论文
共 26 条
[1]  
Adams DG, 2000, PROKARYOTIC DEVELOPMENT, P51
[2]   COMPLEMENTARY CHROMATIC ADAPTATION IN A FILAMENTOUS BLUE-GREEN-ALGA [J].
BENNETT, A ;
BOGORAD, L .
JOURNAL OF CELL BIOLOGY, 1973, 58 (02) :419-435
[3]   C-Phycocyanin:: A potent peroxyl radical scavenger in vivo and in vitro [J].
Bhat, VB ;
Madyastha, KM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 275 (01) :20-25
[4]   Thermophilic C-phycocyanin: effect of temperature, monomer stability, and structure [J].
Edwards, MR ;
Hauer, C ;
Stack, RF ;
Eisele, LE ;
MacColl, R .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1997, 1321 (02) :157-164
[5]   Production of phycocyanin - a pigment with applications in biology, biotechnology, foods and medicine [J].
Eriksen, Niels T. .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 80 (01) :1-14
[6]  
Estrada JEP, 2001, FARMACO, V56, P497
[7]   PHYCOBILIPROTEINS - A FAMILY OF VALUABLE, WIDELY USED FLUOROPHORES [J].
GLAZER, AN .
JOURNAL OF APPLIED PHYCOLOGY, 1994, 6 (02) :105-112
[8]   Anti-inflammatory activity of phycocyanin extract in acetic acid-induced colitis in rats [J].
González, R ;
Rodríguez, S ;
Romay, C ;
Ancheta, O ;
González, A ;
Armesto, J ;
Remirez, D ;
Merino, N .
PHARMACOLOGICAL RESEARCH, 1999, 39 (01) :55-59
[9]   Heat and light stability of three natural blue colorants for use in confectionery and beverages [J].
Jespersen, L ;
Stromdahl, LD ;
Olsen, K ;
Skibsted, LH .
EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2005, 220 (3-4) :261-266
[10]   Nutritional and therapeutic potential of Spirulina [J].
Khan, Z ;
Bhadouria, P ;
Bisen, PS .
CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2005, 6 (05) :373-379