Biologically active protein gradients via microstamping

被引：18

作者：

Bhangale, SM ^{[1
]}

Tjong, V ^{[1
]}

Wu, L ^{[1
]}

Yakovlev, N ^{[1
]}

Moran, PM ^{[1
]}

机构：

[1] Inst Mat Res & Engn, Singapore 117602, Singapore

来源：

ADVANCED MATERIALS | 2005年 / 17卷 / 07期

关键词：

D O I：

10.1002/adma.200400547

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Protein gradients on substrates are prepared using a microstamping technique. An Au pattern is transferred from a patterned silicon stamp to a polymeric substrate, and exposed polymer regions are then selectively activated for protein immobilization. The protein gradient is achieved by varying the spacing between the exposed polymer regions (see Figure).

引用

页码：809 / +

页数：6

共 27 条

[1] AXON GUIDANCE BY GRADIENTS OF A TARGET-DERIVED COMPONENT
BAIER, H
BONHOEFFER, F
[J]. SCIENCE, 1992, 255 (5043) : 472 - 475
[2] Protein patterning
Blawas, AS
Reichert, WM
[J]. BIOMATERIALS, 1998, 19 (7-9) : 595 - 609
[3] CABRAL JMS, 1991, PROTEIN IMMOBILIZATI, pCH3
[4] Gradient micropattern immobilization of EGF to investigate the effect of artificial juxtacrine stimulation
Chen, GP
Ito, YY
[J]. BIOMATERIALS, 2001, 22 (18) : 2453 - 2457
[5] Micrometer-scaled gradient surfaces generated using contact printing of octadecyltrichlorosilane
Choi, SH
Newby, BMZ
[J]. LANGMUIR, 2003, 19 (18) : 7427 - 7435
[6] Nanoimprint lithography
Chou, SY
Krauss, PR
Renstrom, PJ
[J]. JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, 1996, 14 (06): : 4129 - 4133
[7] Nantotechniques and approaches in biotechnology
Curtis, A
Wilkinson, C
[J]. TRENDS IN BIOTECHNOLOGY, 2001, 19 (03) : 97 - 101
[8] Gradients of substrate-bound laminin orient axonal specification of neurons
Dertinger, SKW
Jiang, XY
Li, ZY
Murthy, VN
Whitesides, GM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) : 12542 - 12547
[9] DREYFUS BA, 1966, Patent No. 3230163
[10] Halfter W, 1996, J NEUROSCI, V16, P4389

← 1 2 3 →