Crystallization of mitochondrial respiratory complex II from chicken heart:: a membrane-protein complex diffracting to 2.0 Å

被引：20

作者：

Huang, LS ^{[1
]}

Borders, TM ^{[1
]}

Shen, JT ^{[1
]}

Wang, CJ ^{[1
]}

Berry, EA ^{[1
]}

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Phys Biosci Div, Berkeley, CA 94720 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2005年 / 61卷

关键词：

D O I：

10.1107/S0907444905000181

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure is presented for preparation of diffraction-quality crystals of a vertebrate mitochondrial respiratory complex II. The crystals have the potential to diffract to at least 2.0 angstrom with optimization of post-crystal-growth treatment and cryoprotection. This should allow determination of the structure of this important and medically relevant membrane-protein complex at near-atomic resolution and provide great detail of the mode of binding of substrates and inhibitors at the two substrate-binding sites.

引用

页码：380 / 387

页数：8

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