A simple and rapid method for lithium acetate-mediated transformation of Kluyveromyces marxianus cells

被引：26
作者：
Antunes, DF
de Souza, CG
de Morais, MA
机构：
[1] Univ Fed Pernambuco, Setor Biol Mol LIKA, BR-50670901 Recife, PE, Brazil
[2] Univ Fed Pernambuco, Dept Genet, BR-50670901 Recife, PE, Brazil
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2000年 / 16卷 / 07期
关键词：
dithiothreitol; lithium acetate; Kluyveromyces marxianus; yeast transformation;
D O I：
10.1023/A:1008984403732
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Efficient plasmid transformation of Kluyveromyces marxianus cells of 1.9 x 10(3) transformant mug(-)1 DNA with an episomal plasmid was achieved by the use of a simple lithium acetate method with the addition of 10 mM DTT and an increased heat shock temperature of 47 degreesC. This method is shown to be also efficient for replicative plasmids. Therefore, we suggest its use as a routine method to transform K. marxianus cells.
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收藏
页码：653 / 654
页数：2
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