Non-invasive versus ex vivo measurement of mitochondrial function in an endotoxemia model in rat: Toward monitoring of mitochondrial therapy

被引：13

作者：

Bettink, Mark A. Wefers ^{[1
]}

Harms, Floor A. ^{[1
]}

Dollee, Nathalie ^{[1
]}

Specht, Patricia A. C. ^{[1
]}

Raat, Nicolaas J. H. ^{[1
]}

Schoonderwoerd, G. C. ^{[2
]}

Mik, Egbert G. ^{[1
]}

机构：

[1] Erasmus MC, Dept Anesthesiol, Lab Expt Anesthesiol, S Gravendijkwal 230, NL-3015 CE Rotterdam, Netherlands

[2] Erasmus MC, Dept Clin Genet, Lab Clin Genet, S Gravendijkwal 230, NL-3015 CE Rotterdam, Netherlands

来源：

MITOCHONDRION | 2020年 / 50卷

关键词：

Mitochondria; Endotoxemia; Lipopolysaccharides; Succinic Acid; 5-Aminolevulinic acid; DELAYED FLUORESCENCE; OXYGEN-TENSION; TISSUE OXYGEN; RESPIROMETRY; RESPIRATION;

D O I：

10.1016/j.mito.2019.11.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mitochondrial function has been predominantly measured ex vivo. Due to isolation and preservation procedures ex vivo measurements might misrepresent in vivo mitochondrial conditions. Direct measurement of in vivo mitochondrial oxygen tension (mitoPO(2)) and oxygen disappearance rate (ODR) with the protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) might increase our understanding of mitochondrial dysfunction in the pathophysiology of acute disease. LPS administration decreased mitochondrial respiration (ODR) in vivo but did not alter mitochondrial function as assessed with ex vivo techniques (high resolution respirometry and specific complex determinations). PpIX-TSLT measures in vivo mitoPO(2) and ODR and can be applied non-invasively at the skin.

引用

页码：149 / 157

页数：9

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