Identification of S6K2 as a centrosome-located kinase

被引：28

作者：

Rossi, Rossella

Pester, John M.

McDowell, Mitch

Soza, Samuela

Montecucco, Alessandra

Lee-Fruman, Kay K. ^{[1
]}

机构：

[1] Calif State Univ Long Beach, Dept Biol Sci, Long Beach, CA 90840 USA

[2] CNR, IGM, I-27100 Pavia, Italy

来源：

FEBS LETTERS | 2007年 / 581卷 / 21期

关键词：

ribosomal S6 kinase 2 (S6K2); centrosome; gamma-tubulin;

D O I：

10.1016/j.febslet.2007.07.047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ribosomal S6 kinase 2 (S6K2) acts downstream of the mammalian target of rapamycin (mTOR). Here, we show that some S6K2 localize at the centrosome throughout the cell cycle. S6K2 is found in the pericentriolar area of the centrosome. S6K2 centrosomal localization is unaffected by serum withdrawal or treatment with rapamycin, wortmannin, U0126, or phorbol-12-myristate-13-acetate (PMA). Unlike S6K2, S6 kinase 1 (S6K1) does not localize at the centrosome, suggesting the two kinases may also have nonoverlapping functions. Our data suggest that centrosomal S6K2 may have a role in the phosphoinositide-3-kinase (PI3K)/Akt/mTOR signaling pathway that has also been detected in the centrosome. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：4058 / 4064

页数：7

相关论文

共 28 条

[1] Hamartin, the tuberous sclerosis complex 1 gene product, interacts with polo-like kinase 1 in a phosphorylation-dependent manner [J].

Astrinidis, A ;

Senapedis, W ;

Henske, EP .

HUMAN MOLECULAR GENETICS, 2006, 15 (02) :287-297

[2] P34CDC2 IS LOCATED IN BOTH NUCLEUS AND CYTOPLASM - PART IS CENTROSOMALLY ASSOCIATED AT G2/M AND ENTERS VESICLES AT ANAPHASE [J].

BAILLY, E ;

DOREE, M ;

NURSE, P ;

BORNENS, M .

EMBO JOURNAL, 1989, 8 (13) :3985-3995

[3] DIFFERENTIAL SUBCELLULAR-LOCALIZATION OF 2 ISOFORMS OF P70 S6 PROTEIN-KINASE [J].

COFFER, PJ ;

WOODGETT, JR .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 198 (02) :780-786

[4] Oncogenic tyrosine kinase of malignant hemopathy targets the centrosome [J].

Delaval, B ;

Létard, S ;

Lelièvre, H ;

Chevrier, V ;

Daviet, L ;

Dubreuil, P ;

Birnbaum, D .

CANCER RESEARCH, 2005, 65 (16) :7231-7240

[5]

Filonenko VV, 2004, EXP ONCOL, V26, P294

[6] Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression [J].

Fingar, DC ;

Blenis, J .

ONCOGENE, 2004, 23 (18) :3151-3171

[7] Genes required for mitotic spindle assembly in Drosophila S2 cells [J].

Goshima, Gohta ;

Wollman, Roy ;

Goodwin, Sarah S. ;

Zhang, Nan ;

Scholey, Jonathan M. ;

Vale, Ronald D. ;

Stuurman, Nico .

SCIENCE, 2007, 316 (5823) :417-421

[8] Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase β containing a proline-rich region [J].

Gout, I ;

Minami, T ;

Hara, K ;

Tsujishita, Y ;

Filonenko, V ;

Waterfield, MD ;

Yonezawa, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (46) :30061-30064

[9] PKB/AKT is involved in resumption of meiosis in mouse oocytes [J].

Kalous, J ;

Solc, P ;

Baran, V ;

Kubelka, M ;

Schultz, RM ;

Motlik, J .

BIOLOGY OF THE CELL, 2006, 98 (02) :111-123

[10] PHOSPHOINOSITIDE 3-KINASE BINDS CONSTITUTIVELY TO ALPHA/BETA-TUBULIN AND BINDS TO GAMMA-TUBULIN IN RESPONSE TO INSULIN [J].

KAPELLER, R ;

TOKER, A ;

CANTLEY, LC ;

CARPENTER, CL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (43) :25985-25991