APPLICATION OF OLIGONUCLEOTIDE MICROARRAY FOR THE DETECTION AND GENOTYPING OF CRY GENES IN Bacillus thuringiensis

被引：0
|
作者：
Liu Xu-Guang [1 ,2 ]
Song Fu-Ping [1 ]
Wen Si-Yuan [3 ]
Wang Sheng-Qi [3 ]
Huang Da-Fang [4 ]
Zhang Jie [1 ]
机构：
[1] Inst Plant Protect CAAS, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100094, Peoples R China
[2] Lianyungang Teachers Coll, Lianyungang 222206, Peoples R China
[3] Beijing Inst Radiat Med Sci, Beijing 100850, Peoples R China
[4] Chinese Acad Agr Sci, Inst Biotechnol, Beijing 100081, Peoples R China
来源：
CHILEAN JOURNAL OF AGRICULTURAL RESEARCH | 2010年 / 70卷 / 02期
关键词：
oligonucleotide microarray; cry genes; genotyping; random primer labeling; bioinformatics; STRAINS; PCR; IDENTIFICATION;
D O I：
暂无
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
We have developed a parallel, rapid, high-throughput oligonucleotide microarray-based assay for the reliable detection and genotyping of three cry genes (cry1, cry2 and cry9) in Bacillus thuringiensis (Bt). After the non-polymerase chain reaction (PCR), amplified Bt genomic DNA were fluorescent-labeled using a random primer. The corresponding oligonucleotide probes were designed for the different cry genes that can hybridize Bt genomic DNA after cluster analysis and were printed on glass slides. This microarray has unambiguously detected and identified the cry genes in 10 isolates and reference Bt. Our data demonstrates that the microarray assay is simple and rapid for the detection and genotyping of genes. This type of assay is also a potentially valuable tool for identification and characterization of bacterial functional genes in general.
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收藏
页码：213 / 220
页数：8
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