Interferon Gamma+874T/A Polymorphism Is Associated with Susceptibility to Active Pulmonary Tuberculosis Development in Tunisian Patients

被引:30
作者
Ben Selma, Walid [1 ]
Harizi, Hedi [2 ]
Bougmiza, Iheb [3 ]
Hannachi, Naila [1 ]
Ben Kahla, Imen [1 ]
Zaieni, Radhia [4 ]
Boukadida, Jalel [1 ]
机构
[1] Farhat Hached Univ Hosp, Lab Microbiol & Immunol, UR02SP13, Sousse 4000, Tunisia
[2] Univ Bordeaux 2, CNRS, UMR 5540, F-33076 Bordeaux, France
[3] Fac Med, Dept Community Med, Sousse, Tunisia
[4] Hlth Care Serv, Sousse, Tunisia
关键词
SINGLE NUCLEOTIDE POLYMORPHISM; HUMAN GENETIC SUSCEPTIBILITY; GAMMA-RECEPTOR DEFICIENCY; CALMETTE-GUERIN INFECTION; HUMAN-MODEL; 1ST INTRON; DISEASE; INTERLEUKIN-10; CYTOKINES; RESPONSES;
D O I
10.1089/dna.2010.1157
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Interferon gamma (IFN-gamma) is a key cytokine involved mainly in the defense against intracellular pathogens such as Mycobacterium tuberculosis. Given its key role in the control of tuberculosis (TB), in the present article we have investigated a possible association between IFN-g gene single-nucleotide polymorphism linked to high and low producer phenotypes (IFN-gamma [+874T(high) -> A(low)]) (rs2430561) and risk development of active TB in Tunisian patients. Genomic DNA samples were obtained from 223 patients with active TB (168 pulmonary and 55 extrapulmonary cases) and 150 healthy blood donors. Genotypes were analyzed using polymerase chain reaction-restriction fragment length polymorphism method. The +874 AA genotype (low IFN-gamma producer) was significantly associated with increased risk of developing of active pulmonary TB (odds ratio [OR] = 2.18; 95% confidence intervals [CI], 1.33-3.57; P corrected for the number of genotypes [Pc] = 0.003). By contrast, the AT genotype was found to be significantly associated with resistance to pulmonary TB (OR = 0.46; 95% CI, 0.28-0.74; Pc = 0.0018) and extrapulmonary TB development (OR = 0.46; 95% CI, 0.23-0.91; Pc = 0.045). Collectively, our data showed that the IFN-gamma +874T/A polymorphism is a determinant in the resistance or susceptibility to the development of active TB in the studied population.
引用
收藏
页码:379 / 387
页数:9
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