Synthetic filaments assembled from C-terminally truncated α-synuclein

被引：350

作者：

Crowther, RA

Jakes, R

Spillantini, MG

Goedert, M

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[2] Univ Cambridge, Dept Neurol, Cambridge CB2 2PY, England

来源：

FEBS LETTERS | 1998年 / 436卷 / 03期

关键词：

alpha-synuclein; filament assembly; Parkinson's disease; dementia with lewy bodies; multiple system atrophy;

D O I：

10.1016/S0014-5793(98)01146-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently two point mutations in the alpha-synuclein gene have been found in familial Parkinson's disease. The characteristic fibrous neuropathological lesions of Parkinson's and other neurodegenerative diseases have been shown to stain strongly with antibodies against alpha-synuclein and extracted filaments have been labelled with anti-alpha-synuclein antibodies. In view of the close involvement of alpha-synuclein filaments with pathology, it was important to establish an in vitro assembly system. We report here that C-terminally truncated recombinant alpha-synuclein readily assembles into filaments resembling those isolated from diseased brain and suggest that truncation by proteolysis may play a role in the pathological process, (C) 1998 Federation of European Biochemical Societies.

引用

页码：309 / 312

页数：4

共 22 条

[1]

Baba M, 1998, AM J PATHOL, V152, P879

[2] STRAIGHT AND PAIRED HELICAL FILAMENTS IN ALZHEIMER-DISEASE HAVE A COMMON STRUCTURAL UNIT [J].

CROWTHER, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2288-2292

[3] Neuropathology of Parkinson's disease [J].

Forno, LS .

JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 1996, 55 (03) :259-272

[4] Multiple-system atrophy:: a new α-synuclein disease? [J].

Gai, WP ;

Power, JHT ;

Blumbergs, PC ;

Blessing, WW .

LANCET, 1998, 352 (9127) :547-548

[5] Association of missense and 5′-splice-site mutations in tau with the inherited dementia FTDP-17 [J].

Hutton, M ;

Lendon, CL ;

Rizzu, P ;

Baker, M ;

Froelich, S ;

Houlden, H ;

Pickering-Brown, S ;

Chakraverty, S ;

Isaacs, A ;

Grover, A ;

Hackett, J ;

Adamson, J ;

Lincoln, S ;

Dickson, D ;

Davies, P ;

Petersen, RC ;

Stevens, M ;

de Graaff, E ;

Wauters, E ;

van Baren, J ;

Hillebrand, M ;

Joosse, M ;

Kwon, JM ;

Nowotny, P ;

Che, LK ;

Norton, J ;

Morris, JC ;

Reed, LA ;

Trojanowski, J ;

Basun, H ;

Lannfelt, L ;

Neystat, M ;

Fahn, S ;

Dark, F ;

Tannenberg, T ;

Dodd, PR ;

Hayward, N ;

Kwok, JBJ ;

Schofield, PR ;

Andreadis, A ;

Snowden, J ;

Craufurd, D ;

Neary, D ;

Owen, F ;

Oostra, BA ;

Hardy, J ;

Goate, A ;

van Swieten, J ;

Mann, D ;

Lynch, T .

NATURE, 1998, 393 (6686) :702-705

[6]

Ince PG, 1998, BRAIN PATHOL, V8, P299

[7] Nigral and cortical Lewy bodies and dystrophic nigral neurites in Parkinson's disease and cortical Lewy body disease contain α-synuclein immunoreactivity [J].

Irizarry, MC ;

Growdon, W ;

Gomez-Isla, T ;

Newell, K ;

George, JM ;

Clayton, DF ;

Hyman, BT .

JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 1998, 57 (04) :334-337

[8] IDENTIFICATION OF 2 DISTINCT SYNUCLEINS FROM HUMAN BRAIN [J].

JAKES, R ;

SPILLANTINI, MG ;

GOEDERT, M .

FEBS LETTERS, 1994, 345 (01) :27-32

[9]

JENSEN PH, 1998, IN PRESS J BIOL CHEM

[10] Ala30Pro mutation in the gene encoding α-synuclein in Parkinson's disease [J].

Krüger, R ;

Kuhn, W ;

Müller, T ;

Woitalla, D ;

Graeber, M ;

Kösel, S ;

Przuntek, H ;

Epplen, JT ;

Schöls, L ;

Riess, O .

NATURE GENETICS, 1998, 18 (02) :106-108

← 1 2 3 →