Root exudates modify bacterial diversity of phenanthrene degraders in PAH-polluted soil but not phenanthrene degradation rates
被引:126
作者:
Cebron, Aurelie
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Nancy Univ, CNRS, LIMOS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, LIMOS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Cebron, Aurelie
[1
]
Louvel, Brice
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Nancy Univ, CNRS, LIMOS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, LIMOS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Louvel, Brice
[1
]
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Faure, Pierre
[2
]
France-Lanord, Christian
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CNRS, Ctr Rech Petrog & Geochim, F-54501 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, LIMOS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
France-Lanord, Christian
[3
]
Chen, Yin
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Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, EnglandNancy Univ, CNRS, LIMOS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Chen, Yin
[4
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Murrell, J. Colin
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Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, EnglandNancy Univ, CNRS, LIMOS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Murrell, J. Colin
[4
]
Leyval, Corinne
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Nancy Univ, CNRS, LIMOS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, LIMOS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Leyval, Corinne
[1
]
机构:
[1] Nancy Univ, CNRS, LIMOS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
[2] Nancy Univ, CNRS, Fac Sci,G2R, UMR 7566, F-54506 Vandoeuvre Les Nancy, France
[3] CNRS, Ctr Rech Petrog & Geochim, F-54501 Vandoeuvre Les Nancy, France
[4] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England
P>To determine whether the diversity of phenanthrene-degrading bacteria in an aged polycyclic aromatic hydrocarbon (PAH) contaminated soil is affected by the addition of plant root exudates, DNA stable isotope probing (SIP) was used. Microcosms of soil with and without addition of ryegrass exudates and with 13C-labelled phenanthrene (PHE) were monitored over 12 days. PHE degradation was slightly delayed in the presence of added exudate after 4 days of incubation. After 12 days, 68% of added PHE disappeared both with and without exudate. Carbon balance using isotopic analyses indicated that a part of the 13C-PHE was not totally mineralized as 13CO(2) but unidentified 13C-compounds (i.e. 13C-PHE or 13C-labelled metabolites) were trapped into the soil matrix. Temporal thermal gradient gel electrophoresis (TTGE) analyses of 16S rRNA genes were performed on recovered 13C-enriched DNA fractions. 16S rRNA gene banding showed the impact of root exudates on diversity of PHE-degrading bacteria. With PHE as a fresh sole carbon source, Pseudoxanthomonas sp. and Microbacterium sp. were the major PHE degraders, while in the presence of exudates, Pseudomonas sp. and Arthrobacter sp. were favoured. These two different PHE-degrading bacterial populations were also distinguished through detection of PAH-ring hydroxylating dioxygenase (PAH-RHD(alpha)) genes by real-time PCR. Root exudates favoured the development of a higher diversity of bacteria and increased the abundance of bacteria containing known PAH-RHD(alpha) genes.
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Nancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, France
Nancy Univ, CNRS, LIMOS, UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, France
Biache, Coralie
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Mansuy-Huault, Laurence
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Nancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, France
Mansuy-Huault, Laurence
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Faure, Pierre
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Munier-Lamy, Colette
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Nancy Univ, CNRS, LIMOS, UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, France
Munier-Lamy, Colette
;
Leyval, Corinne
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Nancy Univ, CNRS, LIMOS, UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, France
机构:
Nancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, France
Cebron, Aurelie
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Norini, Marie-Paule
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Nancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, France
Norini, Marie-Paule
;
Beguiristain, Thierry
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Nancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, France
Beguiristain, Thierry
;
Leyval, Corinne
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Nancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, France
机构:
Nancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Cebron, Aurelie
;
Beguiristain, Thierry
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Nancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Beguiristain, Thierry
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Faure, Pierre
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Norini, Marie-Paule
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Nancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Norini, Marie-Paule
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Masfaraud, Jean-Francois
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Univ Paul Verlaine Metz, LIEBE, CNRS, UMR 7146, F-57070 Metz, FranceNancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Masfaraud, Jean-Francois
;
Leyval, Corinne
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Nancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
机构:
Nancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, France
Nancy Univ, CNRS, LIMOS, UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, France
Biache, Coralie
;
Mansuy-Huault, Laurence
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Nancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, France
Mansuy-Huault, Laurence
;
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Faure, Pierre
;
Munier-Lamy, Colette
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Nancy Univ, CNRS, LIMOS, UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, France
Munier-Lamy, Colette
;
Leyval, Corinne
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h-index: 0
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Nancy Univ, CNRS, LIMOS, UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, G2R UMR 7566, F-54506 Vandoeuvre Les Nancy, France
机构:
Nancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, France
Cebron, Aurelie
;
Norini, Marie-Paule
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Nancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, France
Norini, Marie-Paule
;
Beguiristain, Thierry
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Nancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, France
Beguiristain, Thierry
;
Leyval, Corinne
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Nancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, CNRS, Fac Sci,UMR7137, Lab Interact Microorganismes Mineraux Mat Org Sol, F-54506 Vandoeuvre Les Nancy, France
机构:
Nancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Cebron, Aurelie
;
Beguiristain, Thierry
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h-index: 0
机构:
Nancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Beguiristain, Thierry
;
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Faure, Pierre
;
Norini, Marie-Paule
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机构:
Nancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Norini, Marie-Paule
;
Masfaraud, Jean-Francois
论文数: 0引用数: 0
h-index: 0
机构:
Univ Paul Verlaine Metz, LIEBE, CNRS, UMR 7146, F-57070 Metz, FranceNancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
Masfaraud, Jean-Francois
;
Leyval, Corinne
论文数: 0引用数: 0
h-index: 0
机构:
Nancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, FranceNancy Univ, LIMOS, CNRS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France