Root exudates modify bacterial diversity of phenanthrene degraders in PAH-polluted soil but not phenanthrene degradation rates

被引:126
作者
Cebron, Aurelie [1 ]
Louvel, Brice [1 ]
Faure, Pierre [2 ]
France-Lanord, Christian [3 ]
Chen, Yin [4 ]
Murrell, J. Colin [4 ]
Leyval, Corinne [1 ]
机构
[1] Nancy Univ, CNRS, LIMOS, Fac Sci,UMR 7137, F-54506 Vandoeuvre Les Nancy, France
[2] Nancy Univ, CNRS, Fac Sci,G2R, UMR 7566, F-54506 Vandoeuvre Les Nancy, France
[3] CNRS, Ctr Rech Petrog & Geochim, F-54501 Vandoeuvre Les Nancy, France
[4] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England
关键词
POLYCYCLIC AROMATIC-HYDROCARBONS; GRADIENT GEL-ELECTROPHORESIS; CONTAMINATED SOIL; MICROBIAL-DEGRADATION; COMMUNITY STRUCTURE; RIBOSOMAL-RNA; RHIZOSPHERE; BIODEGRADATION; DNA; MICROORGANISMS;
D O I
10.1111/j.1462-2920.2010.02376.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
P>To determine whether the diversity of phenanthrene-degrading bacteria in an aged polycyclic aromatic hydrocarbon (PAH) contaminated soil is affected by the addition of plant root exudates, DNA stable isotope probing (SIP) was used. Microcosms of soil with and without addition of ryegrass exudates and with 13C-labelled phenanthrene (PHE) were monitored over 12 days. PHE degradation was slightly delayed in the presence of added exudate after 4 days of incubation. After 12 days, 68% of added PHE disappeared both with and without exudate. Carbon balance using isotopic analyses indicated that a part of the 13C-PHE was not totally mineralized as 13CO(2) but unidentified 13C-compounds (i.e. 13C-PHE or 13C-labelled metabolites) were trapped into the soil matrix. Temporal thermal gradient gel electrophoresis (TTGE) analyses of 16S rRNA genes were performed on recovered 13C-enriched DNA fractions. 16S rRNA gene banding showed the impact of root exudates on diversity of PHE-degrading bacteria. With PHE as a fresh sole carbon source, Pseudoxanthomonas sp. and Microbacterium sp. were the major PHE degraders, while in the presence of exudates, Pseudomonas sp. and Arthrobacter sp. were favoured. These two different PHE-degrading bacterial populations were also distinguished through detection of PAH-ring hydroxylating dioxygenase (PAH-RHD(alpha)) genes by real-time PCR. Root exudates favoured the development of a higher diversity of bacteria and increased the abundance of bacteria containing known PAH-RHD(alpha) genes.
引用
收藏
页码:722 / 736
页数:15
相关论文
共 71 条
[1]   Analyses of polycyclic aromatic hydrocarbon-degrading bacteria isolated from contaminated soils [J].
Ahn, Y ;
Sanseverino, J ;
Sayler, GS .
BIODEGRADATION, 1999, 10 (02) :149-157
[2]   Aging, bioavailability, and overestimation of risk from environmental pollutants [J].
Alexander, M .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2000, 34 (20) :4259-4265
[3]   Nucleic acid contamination of glycogen used in nucleic acid precipitation and assessment of linear polyacrylamide as an alternative co-precipitant [J].
Bartram, Andrea K. ;
Poon, Calvin ;
Neufeld, Josh D. .
BIOTECHNIQUES, 2009, 47 (06) :1016-1019
[4]   Dynamics and identification of soil microbial populations actively assimilating carbon from 13C-labelled wheat residue as estimated by DNA- and RNA-SIP techniques [J].
Bernard, Laetitia ;
Mougel, Christophe ;
Maron, Pierre-Alain ;
Nowak, Virginie ;
Leveque, Jean ;
Henault, Catherine ;
Haichar, Feth el Zahar ;
Berge, Odile ;
Marol, Christine ;
Balesdent, Jerome ;
Gibiat, Frederic ;
Lemanceau, Philippe ;
Ranjard, Lionel .
ENVIRONMENTAL MICROBIOLOGY, 2007, 9 (03) :752-764
[5]   Effects of thermal desorption on the composition of two coking plant soils: Impact on solvent extractable organic compounds and metal bioavailability [J].
Biache, Coralie ;
Mansuy-Huault, Laurence ;
Faure, Pierre ;
Munier-Lamy, Colette ;
Leyval, Corinne .
ENVIRONMENTAL POLLUTION, 2008, 156 (03) :671-677
[6]   Fate of polycyclic aromatic hydrocarbons (PAH) in the rhizosphere and mycorrhizosphere of ryegrass [J].
Binet, P ;
Portal, JM ;
Leyval, C .
PLANT AND SOIL, 2000, 227 (1-2) :207-213
[7]   Real-Time PCR quantification of PAH-ring hydroxylating dioxygenase (PAH-RHDα) genes from Gram positive and Gram negative bacteria in soil and sediment samples [J].
Cebron, Aurelie ;
Norini, Marie-Paule ;
Beguiristain, Thierry ;
Leyval, Corinne .
JOURNAL OF MICROBIOLOGICAL METHODS, 2008, 73 (02) :148-159
[8]   Influence of Vegetation on the In Situ Bacterial Community and Polycyclic Aromatic Hydrocarbon (PAH) Degraders in Aged PAH-Contaminated or Thermal-Desorption-Treated Soil [J].
Cebron, Aurelie ;
Beguiristain, Thierry ;
Faure, Pierre ;
Norini, Marie-Paule ;
Masfaraud, Jean-Francois ;
Leyval, Corinne .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (19) :6322-6330
[9]  
Cerniglia Carl E., 1992, Biodegradation, V3, P351, DOI 10.1007/BF00129093
[10]   Copper availability and bioavailability are controlled by rhizosphere pH in rape grown in an acidic Cu-contaminated soil [J].
Chaignon, Valerie ;
Quesnoit, Marie ;
Hinsinger, Philippe .
ENVIRONMENTAL POLLUTION, 2009, 157 (12) :3363-3369