Rapid and Quantitative Measurement of Single Quantum Dots in a Sheath Flow Cuvette

被引：7

作者：

Wang, Shuo ^{[1
]}

Li, Lihong ^{[1
]}

Jin, Shenghao ^{[1
]}

Li, Weifeng ^{[1
]}

Hang, Wei ^{[1
]}

Yan, Xiaomei ^{[1
]}

机构：

[1] Xiamen Univ, Collaborat Innovat Ctr Chem Energy Mat, MOE Key Lab Spectrochem Anal & Instrumentat, Key Lab Chem Biol Fujian Prov,Dept Chem Biol,Coll, Xiamen 361005, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 18期

基金：

中国国家自然科学基金;

关键词：

SEMICONDUCTOR NANOCRYSTALS; PARTICLE TRACKING; AQUEOUS-SOLUTION; DARK FRACTION; METAL-IONS; IN-VIVO; FLUORESCENCE; QUANTIFICATION; BLINKING; CDSE;

D O I：

10.1021/acs.analchem.7b01885

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Semiconducting quantum dots (QDs) are finding a wide range of biomedical applications due to their intense fluorescence brightness and long-term photostability. Here, we report precise quantification of the fluorescence intensity of single QDs on a laboratory-built high-sensitivity flow cytometer (HSFCM). The nearly uniform illumination of the particles at the intense portions of the radiation field resulted in narrowly distributed signals with high signal-to-noise ratios. By analysis of thousands of QDs individually in as little time as 1 min, intrinsic polydispersity was quickly revealed in a statistically robust manner. Applications of this technique in QD quality assessment, study of metal ion influence, and evaluation of aggregation upon biomolecule coupling are presented. Moreover, an accurate measurement of the QD particle concentration was achieved via single-particle enumeration. HSFCM is believed to provide a powerful characterization tool for QD synthesis and application development.

引用

页码：9857 / 9863

页数：7

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