Extension of life-span using a RNAi model and in vivo antioxidant effect of Opuntia fruit extracts and pure betalains in Caenorhabditis elegans

被引:63
作者
Alejandra Guerrero-Rubio, M. [1 ]
Hernandez-Garcia, Samanta [1 ]
Garcia-Carmona, Francisco [1 ]
Gandia-Herrero, Fernando [1 ]
机构
[1] Univ Murcia, Dept Bioquim & Biol Mol A, Unidad Docente Biol, Fac Vet, Reg Campus Int Excellence Campus Mare Nostrum, Murcia, Spain
关键词
C; elegans; Lifespan; Antioxidant; In vivo; Green fluorescent protein; STRESS-RESPONSE; FLUORESCENCE; RESISTANCE; PIGMENTS; BETANIN;
D O I
10.1016/j.foodchem.2018.09.067
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Betalains are nitrogenous plant pigments known for their high antioxidant capacity. For the first time, this antioxidant nature has been studied in an in vivo system using the model organism Caenorhabditis elegans. The oxidative stress caused in the fluorescent strain TJ375 (hsp-16.2::GFP) was reversed by the presence of both natural and semi-synthetic betalains, with an ED50 value around 25 mu M for betacyanins and up to 10 mu M for betaxanthins, with indicaxanthin, the major pigments in prickly pear fruits, as the most effective betalain. The effect of model betalains on the lifespan of the wild-type N2 strain was carefully studied using the automatic platform "Lifespan Machine". In a search for different approaches to suppress progeny, pop-1 RNAi was used to avoid FUdR use. The presence of betalains in the medium, both as pure compounds and as enriched Opuntia extracts significantly increased the lifespan of C. elegans.
引用
收藏
页码:840 / 847
页数:8
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