DETERMINATION OF ADENOSINE AND INOSINE IN SHEEP PLASMA USING SOLID PHASE EXTRACTION FOLLOWED BY LIQUID CHROMATOGRAPHY WITH UV DETECTION

被引：2

作者：

Chitta, Rajesh ^{[1
]}

Pendela, Murali ^{[1
]}

Yekkala, Raja ^{[1
]}

Herijgers, Paul ^{[2
]}

Hoogmartens, Jos ^{[1
]}

Adams, Erwin ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Farmaceut Anal, B-3000 Louvain, Belgium

[2] Katholieke Univ Leuven, Afdeling Expt Cardiale Heelkunde, UZ Gasthuisberg, B-3000 Louvain, Belgium

来源：

ANALYTICAL LETTERS | 2010年 / 43卷 / 14期

关键词：

Adenosine; Inosine; Liquid chromatography; Sheep plasma; SPE; HYPOXANTHINE; ISCHEMIA; BLOOD;

D O I：

10.1080/00032711003717323

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and sensitive liquid chromatography assay following solid phase extraction was developed for simultaneous determination of adenosine and inosine in sheep plasma. The system consisted of a Symmetry C-18 column, a mobile phase composed of acetonitrile, 100 mM sodium dihydrogen phosphate and water, and ultraviolet detection at 254 nm. The method showed good sensitivity (limits of detection for adenosine and inosine were 30 and 50 ng/ml, respectively, in the plasma samples), repeatability, and linearity. The developed method was applied to sheep plasma samples from a study examining the cardio active potential of the combination of adenosine and inosine.

引用

页码：2267 / 2274

页数：8