Reveals of candidate active ingredients in Justicia and its anti-thrombotic action of mechanism based on network pharmacology approach and experimental validation

被引：14

作者：

Hong, Zongchao ^{[1
]}

Zhang, Ting ^{[2
]}

Zhang, Ying ^{[1
]}

Xie, Zhoutao ^{[1
]}

Lu, Yi ^{[1
]}

Yao, Yunfeng ^{[1
]}

Yang, Yanfang ^{[1
,3
,4
]}

Wu, Hezhen ^{[1
,3
,4
]}

Liu, Bo ^{[1
,3
]}

机构：

[1] Hubei Univ Chinese Med, Fac Pharm, Wuhan 430065, Peoples R China

[2] Hubei Univ Chinese Med, Hubei Hosp Tradit Chinese Med, Affiliated Hosp, Wuhan, Peoples R China

[3] Key Lab Tradit Chinese Med Resources & Chem Hubei, Wuhan, Peoples R China

[4] Collaborat Innovat Ctr Tradit Chinese Med New Pro, Wuhan, Peoples R China

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

PLATELET; THROMBOSIS; AGGREGATION; ACTIVATION; EXPRESSION; APOPTOSIS; ACID;

D O I：

10.1038/s41598-021-96683-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Thrombotic diseases seriously threaten human life. Justicia, as a common Chinese medicine, is usually used for anti-inflammatory treatment, and further studies have found that it has an inhibitory effect on platelet aggregation. Therefore, it can be inferred that Justicia can be used as a therapeutic drug for thrombosis. This work aims to reveal the pharmacological mechanism of the anti-thrombotic effect of Justicia through network pharmacology combined with wet experimental verification. During the analysis, 461 compound targets were predicted from various databases and 881 thrombus-related targets were collected. Then, herb-compound-target network and protein-protein interaction network of disease and prediction targets were constructed and cluster analysis was applied to further explore the connection between the targets. In addition, Gene Ontology (GO) and pathway (KEGG) enrichment were used to further determine the association between target proteins and diseases. Finally, the expression of hub target proteins of the core component and the anti-thrombotic effect of Justicia's core compounds were verified by experiments. In conclusion, the core bioactive components, especially justicidin D, can reduce thrombosis by regulating F2, MMP9, CXCL12, MET, RAC1, PDE5A, and ABCB1. The combination of network pharmacology and the experimental research strategies proposed in this paper provides a comprehensive method for systematically exploring the therapeutic mechanism of multi-component medicine.

引用

页数：18

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