Role of oxidative stress in disrupting the function of negative glucocorticoid response element in daily amphetamine-treated rats

被引：7
作者：
Chu, Shu-Chen [1 ]
Yu, Ching-Han [2 ,3 ]
Chen, Pei-Ni [3 ,4 ]
Hsieh, Yih-Shou [3 ,4 ]
Kuo, Dong-Yih [2 ,3 ]
机构：
[1] Cent Taiwan Univ Sci & Technol, Dept Food Sci, Taichung 406, Taiwan
[2] Chung Shan Med Univ, Dept Physiol, Taichung 40201, Taiwan
[3] Chung Shan Med Univ Hosp, Taichung 40201, Taiwan
[4] Chung Shan Med Univ, Inst Biochem & Biotechnol, Taichung 40201, Taiwan
来源：
PSYCHONEUROENDOCRINOLOGY | 2016年 / 71卷
关键词：
Oxidative stress; Glucocorticoid response element; NPY; POMC; Brain; MEDIATED APPETITE CONTROL; NEUROPEPTIDE-Y NPY; INDUCED CORTICOSTERONE RELEASE; FOOD-INTAKE; FEEDING-BEHAVIOR; BLOOD-GLUCOSE; DOPAMINE D-1; RECEPTOR; BRAIN; METHAMPHETAMINE;
D O I：
10.1016/j.psyneuen.2016.04.025
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Amphetamine (AMPH)-induced appetite suppression is associated with changes in hypothalamic reactive oxygen species (ROS), antioxidants, neuropeptides, and plasma glucocorticoid. This study explored whether ROS and glucocorticoid response element (GRE), which is the promoter site of corticotropin-releasing hormone (CRH) gene, participated in neuropeptides-mediated appetite control. Rats were treated daily with AMPH for four days, and changes in food intake, plasma glucocorticoid and expression levels of hypothalamic neuropeptide Y (NPY), proopiomelanocortin (POMC), superoxide dismutase (SOD), CRH, and glucocorticoid receptor (GR) were examined and compared. Results showed that food intake decreased and NPY gene down-regulated, while POMC, SOD, and CRH gene up-regulated during AMPH treatment. GR and GRE-DNA bindings were disrupted on Day 1 and Day 2 when glucocorticoid levels were still high. Pretreatment, with GR inhibitor or ROS scavenger modulated mRNA levels in NPY, POMC, SOD and CRH in AMPH-treated rats. We suggest that disruptions of negative GRE (nGRE) on Day 1 and Day 2 are associated with an increase in oxidative stress during the regulation of NPY/POMC-mediated appetite control in AMPH-treated rats. These results advance the understanding of molecular mechanism in regulating AMPH-mediated appetite suppression. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1 / 11
页数：11
相关论文
共 53 条
[1]   Role of Prefrontal Dopaminergic Neurotransmission in Glucocorticoid Receptor-Mediated Modulation of Methamphetamine-Induced Hyperactivity [J].
Ago, Yukio ;
Arikawa, Shinsuke ;
Yata, Miyuki ;
Yano, Koji ;
Abe, Michikazu ;
Takuma, Kazuhiro ;
Matsuda, Toshio .
SYNAPSE, 2009, 63 (01) :7-14
[2]   Activation of the hypothalamic-pituitary-adrenal axis by addictive drugs: different pathways, common outcome [J].
Armario, Antonio .
TRENDS IN PHARMACOLOGICAL SCIENCES, 2010, 31 (07) :318-325
[3]  
Aynara Wulsin C., 2010, PSYCHONEUROENDOCRINO, V35, P1100
[4]   Role for mitochondrial reactive oxygen species in brain lipid sensing:: Redox regulation of food intake [J].
Benani, Alexandre ;
Troy, Stephanie ;
Carmona, Maria Carmen ;
Fioramonti, Xavier ;
Lorsignol, Anne ;
Leloup, Corinne ;
Casteilla, Louis ;
Penicaud, Luc .
DIABETES, 2007, 56 (01) :152-160
[5]   Neurotoxicity of substituted amphetamines: Molecular and cellular mechanisms [J].
Cadet, Jean Lud ;
Krasnova, Irina N. ;
Jayanthi, Subramaniam ;
Lyles, Johnalyn .
NEUROTOXICITY RESEARCH, 2007, 11 (3-4) :183-202
[6]   Enhanced Upregulation of CRH mRNA Expression in the Nucleus Accumbens of Male Rats after a Second Injection of Methamphetamine Given Thirty Days Later [J].
Cadet, Jean Lud ;
Brannock, Christie ;
Ladenheim, Bruce ;
McCoy, Michael T. ;
Krasnova, Irina N. ;
Lehrmann, Elin ;
Becker, Kevin G. ;
Jayanthi, Subramaniam .
PLOS ONE, 2014, 9 (01)
[7]   Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line [J].
Chen, Pei-Ni ;
Chu, Shu-Chen ;
Chiou, Hui-Ling ;
Kuo, Wu-Hsien ;
Chiang, Chui-Liang ;
Hsieh, Yih-Shou .
CANCER LETTERS, 2006, 235 (02) :248-259
[8]   Evidence for the involvement of dopamine D1 and D2 receptors in mediating the decrease of food intake during repeated treatment with amphetamine [J].
Chen, TY ;
Duh, SL ;
Huang, CC ;
Lin, TB ;
Kuo, DY .
JOURNAL OF BIOMEDICAL SCIENCE, 2001, 8 (06) :462-466
[9]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[10]   The role of stress and the hypothalamic-pituitary-adrenal axis in the pathogenesis of the metabolic syndrome: neuro-endocrine and target tissue-related causes [J].
Chrousos, GP .
INTERNATIONAL JOURNAL OF OBESITY, 2000, 24 (Suppl 2) :S50-S55
123456