Effects of agitation and aeration on the production of extracellular glucose oxidase from a recombinant Saccharomyces cerevisiae

被引：19

作者：

Kapat, A ^{[1
]}

Jung, JK ^{[1
]}

Park, YH ^{[1
]}

Hong, SY ^{[1
]}

Choi, HK ^{[1
]}

机构：

[1] Doosan Training & Technol Ctr, Biotechnol Grp, Yong In 449840, Kyonggi, South Korea

来源：

BIOPROCESS ENGINEERING | 1998年 / 18卷 / 05期

关键词：

D O I：

10.1007/PL00008994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant strain of Saccharomyces cerevisiae, GAL-GO2, was developed to facilitate the production of extracellular glucose oxidase (GOD). The recombinant strain secreted 85% (8.7 U/ml) of the total GOD (10.3 U/ml) produced in shake flask culture. For further enhancement of GOD production, optimization of the speed of agitation and the rate of aeration in a stirred tank fermentor was carried out. Response surface methodology with appropriate statistical experimental design was employed for this purpose. The maximal level of extracellular GOD was achieved when the speed of agitation and the rate of aeration were 420 rpm and 0.25 vvm, respectively. The enzyme production was increased by 74% compared to the level obtained under unoptimized conditions.

引用

页码：347 / 351

页数：5

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