Effects of glucose concentration, medium osmotic pressure and light intensity on the growth of Marchantia paleacea var. diptera cells in photomixotrophic culture

Batch cultures of photomixotrophic cells of Marchantia paleacea var, diptera were conducted at 25 degrees C under the initial conditions of glucose concentration of 20 or 40 kg/m(3), medium osmotic pressure of 520 or 830 kPa and mean incident light intensity of 0, 13 or 28 W/m(2). The glucose concentration, osmotic pressure and mean light intensity in medium declined with increasing cell mass concentration, so that they became limiting factors against the cell growth during the cultures. A kinetic expression for the cell growth was presented by considering the effects of three limiting factors, and applied to the data obtained from the batch cultures. The calculation results fitted closely with the experimental data in the cultures.