Transmembrane protein 173 inhibits RANKL-induced osteoclast differentiation

被引：14

作者：

Choe, Chung-Hyeon ^{[1
,2
,3
]}

Park, In Sun ^{[1
]}

Park, Jisang ^{[4
,5
]}

Yu, Kang-Yeol ^{[1
]}

Jang, Hyonseok ^{[6
]}

Kim, Ju ^{[1
]}

Jang, Yong-Suk ^{[2
,3
,4
,5
]}

机构：

[1] Jeonju Biomat Inst, Jeonju 561360, South Korea

[2] Chonbuk Natl Univ, Dept Mol Biol, Jeonju 561756, South Korea

[3] Chonbuk Natl Univ, Inst Mol Biol & Genet, Jeonju 561756, South Korea

[4] Chonbuk Natl Univ, Dept Bioact Mat Sci, Jeonju 561756, South Korea

[5] Chonbuk Natl Univ, Res Ctr Bioact Mat, Jeonju 561756, South Korea

[6] Korea Univ Ansan Hosp, Dept Oral & Maxillofacial Surg, Ansan 425707, South Korea

来源：

FEBS LETTERS | 2015年 / 589卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

Osteoclastogenesis; RANK ligand; Transmembrane protein 173; Tartrate-resistant acid phosphatase; GENE-EXPRESSION; BONE; OSTEOPOROSIS; ACTIVATION; IMMUNE; ACID; OSTEOIMMUNOLOGY; OSTEOPROTEGERIN; TRANSDUCTION; DENSITY;

D O I：

10.1016/j.febslet.2015.02.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tmem173 was identified as a growth inhibitor associated with major histocompatibility complex (MHC) class II and a potential stimulator for IFN-beta, an innate immune inducer and a negative feedback controller for RANKL-induced osteoclast differentiation of monocytic macrophage cells. In this study, we confirmed that transmembrane protein 173 (Tmem173) overexpression inhibited the expression of osteoclast-specific genes, tartrate-resistant acid phosphatase (TRAP), cathepsin K, and matrix metalloproteinase-9 (MMP-9), as well as bone resorption pit formation in RANKL-treated RAW 264.7 cells. Activation of osteoclast-specific transcription factors, c-Fos and nuclear factor of activated T cells cytoplasmic-1 (NFATc1), and RANKL-induced activation of ERK were also down-regulated by Tmem173 overexpression. Collectively, these results suggest that Tmem173 plays a regulatory role in RANKL-RANK-mediated signaling in osteoclastogenesis. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：836 / 841

页数：6

共 27 条

[1] Mechanisms of interferon-β effects on bone homeostasis [J].