Development of microsatellite markers in fig (Ficus carica L.)

被引：1
|
作者：
Giraldo, E [1 ]
López-Corrales, M [1 ]
Viruel, MA [1 ]
Hormaza, JI [1 ]
机构：
[1] SIDT, Badajoz 06187, Spain
来源：
Proceedings of the XIth Eucarpia Symposium on Fruit Breeding and Genetics, Vols 1 and 2 | 2004年 / 663期
关键词：
germplasm; cultivar identification; molecular markers; Moraceae;
D O I：
10.17660/ActaHortic.2004.663.113
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The fig germplasm collection of Badajoz (Spain) established in 1985 contains 215 accessions obtained from different geographical areas. To improve the management of germplasm collections we started the process of finding microsatellites or SSRs in this species. We report here the results obtained with 8 primer pairs of microsatellites obtained from a CT-enriched genomic library of the Spanish fig cultivar Cuello de Dama Blanco. The SSRs obtained were evaluated with an automatic sequencer in a set of 15 fig cultivars coming from different countries such as USA, France, Israel, Portugal, Spain and Turkey. The number of alleles per locus ranged from 2 to 4 with a mean value of 2.75. Moreover, cross-species amplification was tested with 14 Ficus and 2 Morus species. Five of the microsatellite primer pairs produced amplification fragments with all the Ficus species and all of them with Morus alba.
引用
收藏
页码：635 / 638
页数：4
相关论文
共 50 条
  • [31] Genetic diversity of fig (Ficus carica L.) germplasm from the Mediterranean basin as revealed by SSR markers
    Sclavounos, Athanasios
    Roussos, Petros
    Milla, Sotiria
    Kostas, Panagiotis
    Samaras, Yiannis
    Pozzi, Carlo
    Molla, Johiruddin
    Chitikineni, Annapurna
    Varshney, Rajeev K.
    Voloudakis, Andreas
    GENETIC RESOURCES AND CROP EVOLUTION, 2023, 70 (05) : 1395 - 1406
  • [32] Agromorphological characterization of tradicional Spanish fig (Ficus carica L.) cultivars
    Perez-Sanchez, R.
    Morales-Corts, M. R.
    Gomez-Sanchez, M. A.
    VII CONGRESO IBERICO DE AGROINGENIERIA Y CIENCIAS HORTICOLAS: INNOVAR Y PRODUCIR PARA EL FUTURO. INNOVATING AND PRODUCING FOR THE FUTURE, 2014, : 495 - 500
  • [33] Propagation of fig by hardwood cuttings in the field conditions (Ficus carica L.)
    Tekintas, FE
    Seferoglu, G
    FIRST INTERNATIONAL SYMPOSIUM ON FIG, 1998, (480): : 119 - 120
  • [34] Micropropagation of fig (Ficus carica L.) 'Roxo de Valinhos' plants
    Fráguas, CB
    Pasqual, M
    Dutra, LF
    Cazetta, JO
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2004, 40 (05) : 471 - 474
  • [35] Determination of genetic diversity among the fig (Ficus carica L.) genotypes using AFLP and SSR markers
    Yilmaz, Y.
    Ikten, H.
    IV BALKAN SYMPOSIUM ON FRUIT GROWING, 2020, 1289 : 281 - 289
  • [36] Multivariate analysis of fig (Ficus carica L.) germplasm in southern Tunisia
    Mars, M
    Chebli, T
    Marrakchi, M
    FIRST INTERNATIONAL SYMPOSIUM ON FIG, 1998, (480): : 75 - 81
  • [37] Rooting of fig (Ficus carica L.) cuttings:: Cutting time and IBA
    Chalfun, NNJ
    Pasqual, M
    Norberto, PM
    Dutra, LF
    Cavalcante-Alves, JM
    PROCEEDINGS OF THE 2ND INTERNATIONAL SYMPOSIUM ON FIG, 2003, (605): : 137 - 140
  • [38] Selection studies on fig (Ficus carica L.) in Antalya Province of Turkey
    Gozlekci, S.
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2010, 9 (46): : 7857 - 7861
  • [39] Genotype authenticity of the major fig (Ficus carica L.) cultivars in Croatia
    Ivkovic, Luka
    Bandelj, Dunja
    Arbeiter, Alenka Baruca
    Fruk, Goran
    Pejic, Ivan
    JOURNAL OF CENTRAL EUROPEAN AGRICULTURE, 2024, 25 (04): : 988 - 1004
  • [40] Nutrition of fig (Ficus carica L.) under hydroponics and greenhouse conditions
    Manuel Mendoza-Castillo, Victor
    Pineda-Pineda, Joel
    Manuel Vargas-Canales, Juan
    Hernandez-Arguello, Eduardo
    JOURNAL OF PLANT NUTRITION, 2019, 42 (11-12) : 1350 - 1365
12345