NMR of large (>25 kDa) proteins and protein complexes

被引：27
作者：
Arrowsmith, CH
Wu, YS
机构：
[1] Univ Toronto, Ontario Canc Inst, Div Mol & Struct Biol, Toronto, ON M5G 2M9, Canada
[2] Univ Toronto, Dept Med Biophys, Toronto, ON M5G 2M9, Canada
来源：
PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY | 1998年 / 32卷 / 03期
关键词：
NMR spectroscopy; deuterium labeling; isotope labeling; isotope assisted NMR;
D O I：
10.1016/S0079-6565(98)00017-X
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
NMR spectroscopy is currently a major technique for the determination of 3D structures of proteins and protein complexes smaller than 20 kDa. We discuss recent advances in isotope labeling strategies and NMR pulse sequences that together establish methods for extending the utility of NMR spectroscopy to proteins and complexes significantly larger than 25 kDa. (C) 1998 Elsevier Science B.V.
引用
收藏
页码：277 / 286
页数：10
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