Traffic of leukocytes and cytokine up-regulation in the central nervous system in sepsis

被引：81

作者：

Comim, Clarissa M. ^{[2
]}

Vilela, Marcia C. ^{[3
]}

Constantino, Larissa S. ^{[1
]}

Petronilho, Fabricia ^{[1
]}

Vuolo, Franciele ^{[1
]}

Lacerda-Queiroz, Norinne ^{[3
]}

Rodrigues, David H. ^{[3
]}

da Rocha, Joao Luiz ^{[1
]}

Teixeira, Antonio L. ^{[3
]}

Quevedo, Joao ^{[2
]}

Dal-Pizzol, Felipe ^{[1
]}

机构：

[1] Univ Extremo Sul Catarinense, Programa Posgrad Ciencias Saude, Inst Nacl Ciencia & Tecnol Translac Med, Lab Fisiopatol Expt,Unidade Acad Ciencias Saude, BR-88806000 Criciuma, SC, Brazil

[2] Univ Extremo Sul Catarinense, Programa Posgrad Ciencias Saude, Inst Nacl Ciencia & Tecnol Translac Med, Lab Neurociencias,Unidade Acad Ciencias Saude, BR-88806000 Criciuma, SC, Brazil

[3] Univ Fed Minas Gerais, Lab Imunofarmacol, Dept Bioquim & Imunol, Inst Ciencias Biol, BR-31270901 Belo Horizonte, MG, Brazil

来源：

INTENSIVE CARE MEDICINE | 2011年 / 37卷 / 04期

关键词：

Sepsis; Central nervous system; Brain microvasculature; Delirium; BLOOD-BRAIN-BARRIER; CECAL LIGATION; EXOCYTOSIS;

D O I：

10.1007/s00134-011-2151-2

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

Purpose: To evaluate the effects of sepsis on brain microvasculature leukocyte rolling and adherence, myeloperoxidase (MPO) activity, cytokine and chemokine concentrations, and behavioral screening 6, 12, and 24 h after sepsis induction. Methods: C57BL/6 mice or Wistar rats underwent cecal ligation and perforation (CLP) or sham operation. At 6, 12, and 24 h after sepsis induction, intravital microscopy was performed in the mice brain microvasculature to evaluate leukocyte rolling and adherence. Animals were killed and had the brain removed to determine MPO activity and the levels of cytokines and chemokines. A behavioral screening was also performed in a separate cohort of animals. Blood-brain barrier (BBB) permeability and cytokines and chemokines were determined in different brain regions in Wistar rats. Results: There was a decrease in circulating leukocyte levels at 6, 12, and 24 h, an increase in rolling and adhesion of leukocytes in the brain microvasculature, followed by an increase in brain MPO activity. In addition, there was an increase in both brain cytokines and chemokines at different times. There was a decrease in the neuropsychiatric state muscle tone and strength only at 6 h, and a decrease in the autonomous function at 6 and 12 h. The pattern of brain cytokines and chemokines, and BBB permeability between the analyzed regions seemed to be similar with minor differences. Conclusions: During sepsis the brain's production of cytokines and chemokines is an early event and it seemed to participate both in central nervous system (CNS) dysfunction and BBB permeability alterations, reinforcing the role of brain inflammatory response in the acute CNS dysfunction associated with sepsis.

引用

页码：711 / 718

页数：8

共 25 条

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