Synthesis of polymer-coated silica particles with specific recognition sites for glucose oxidase by the molecular imprinting technique

被引:43
作者
Hirayama, K [1 ]
Burow, M [1 ]
Morikawa, Y [1 ]
Minoura, N [1 ]
机构
[1] Natl Inst Mat & Chem Res, Dept Organ Chem, Tsukuba, Ibaraki 3058565, Japan
关键词
D O I
10.1246/cl.1998.731
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A polymer layer around silica beads, composed of acrylamide, acrylic acid, and crosslinkers, was able to preferentially recognize glucose oxidase (GOD) from a mixture of proteins. Matching between the zeta-potential of GOD and that of its imprinted polymer is suggested to be important in enhancing the recognition effect.
引用
收藏
页码:731 / 732
页数:2
相关论文
共 6 条
[1]   Molecular imprinting: Synthesis of polymer particles with antibody-like binding characteristics for glucose oxidase [J].
Burow, M ;
Minoura, N .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 227 (02) :419-422
[2]  
Guilbault G.G., 1976, HDB ENZYMATIC METHOD, P106
[3]  
KEMPE M, 1995, J MOL RECOGNIT, V8, P35, DOI 10.1002/jmr.300080106
[4]   INFLUENCE OF PROTEIN ON POLYSILOXANE POLYMER FORMATION - EVIDENCE FOR INDUCTION OF COMPLEMENTARY PROTEIN-POLYMER INTERACTIONS [J].
VENTON, DL ;
GUDIPATI, E .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1995, 1250 (02) :126-136
[5]   DRUG ASSAY USING ANTIBODY MIMICS MADE BY MOLECULAR IMPRINTING [J].
VLATAKIS, G ;
ANDERSSON, LI ;
MULLER, R ;
MOSBACH, K .
NATURE, 1993, 361 (6413) :645-647
[6]   MOLECULAR IMPRINTING IN CROSS-LINKED MATERIALS WITH THE AID OF MOLECULAR TEMPLATES - A WAY TOWARDS ARTIFICIAL ANTIBODIES [J].
WULFF, G .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1995, 34 (17) :1812-1832