MIR4435-2HG regulates cancer cell behaviors in oral squamous cell carcinoma cell growth by upregulating TGF-β1

被引：24
作者：
Shen, Huan [1 ]
Sun, Bin [1 ]
Yang, Yongjin [1 ]
Cai, Xingwei [1 ]
Bi, Lixia [1 ]
Deng, Lin [1 ]
Zhang, Luyue [2 ]
机构：
[1] PLA Rocket Force Gen Hosp, Dept Stomatol, Beijing 100088, Peoples R China
[2] Fourth Mil Med Univ, Sch Basic Med Sci, 147 West Changle Rd, Xian 710032, Shannxi, Peoples R China
来源：
ODONTOLOGY | 2020年 / 108卷 / 04期
关键词：
Oral squamous cell carcinoma; MIR4435-2HG; TGF-beta; 1; Proliferation; Migration; BETA; METASTASIS; PROMOTES;
D O I：
10.1007/s10266-020-00488-x
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
MIR4435-2HG has been characterized as an oncogenic lncRNA in several types of cancer, while its role in oral squamous cell carcinoma (OSCC, a major subtype of oral cancer) has not been characterized. We explored the functionality of MIR4435-2HG in OSCC and investigated its interactions with TGF-beta 1. Blood samples were extracted from OSCC patients (n = 44) and healthy volunteers (n = 38), RT-qPCR, CCK-8, Transwell assays and western blot were performed in this study. The results showed that levels of MIR4435-2HG and TGF-beta 1 in plasma were upregulated in OSCC. Across OSCC plasma samples, TGF-beta 1 and MIR4435-2HG were significantly and positively correlated. Overexpression of MIR4435-2HG resulted in upregulated TGF-beta 1 expression, while exogenous TGF-beta 1 treatment had no effect on the expression of MIR4435-2HG. Overexpression of MIR4435-2HG and exogenous TGF-beta 1 treatment led to promoted, while TGF-beta inhibitor led to inhibited migration, proliferation and invasion of cancer cells. Moreover, TGF-beta inhibitor led to reduced effects of overexpressing MIR4435-2HG. Therefore, MIR4435-2HG regulates the behaviors of OSCC cells by promoting the expression of TGF-beta 1.
引用
收藏
页码：553 / 559
页数：7
相关论文
共 22 条
  • [1] [Anonymous], 2014, BIOMED RES INT
  • [2] Oral cavity and pharynx cancer incidence rates in the United States, 1975-1998
    Canto, MT
    Devesa, SS
    [J]. ORAL ONCOLOGY, 2002, 38 (06): : 610 - 617
  • [3] The high-risk HPV oncogene E7 upregulates miR-182 expression through the TGF-β/Smad pathway in cervical cancer
    Chen, Jiao
    Deng, Yan
    Ao, Liangfei
    Song, Yi
    Xu, Yan
    Wang, Chi Chiu
    Choy, Kwong Wai
    Chung, Kwok Hung Tony
    Du, Quan
    Sui, Yi
    Yang, Tao
    Yang, Jing
    Li, Hu
    Zou, Chang
    Tang, Tao
    [J]. CANCER LETTERS, 2019, 460 : 75 - 85
  • [4] Up-regulation of miR-455-5p by the TGF--SMAD signalling axis promotes the proliferation of oral squamous cancer cells by targeting UBE2B
    Cheng, Chao-Min
    Shiah, Shine-Gwo
    Huang, Chien-Chang
    Hsiao, Jenn-Ren
    Chang, Jang-Yang
    [J]. JOURNAL OF PATHOLOGY, 2016, 240 (01) : 38 - 49
  • [5] Cancer-associated fibroblasts regulate keratinocyte cell-cell adhesion via TGF-β-dependent pathways in genotype-specific oral cancer
    Cirillo, N.
    Hassona, Y.
    Celentano, A.
    Lim, K. P.
    Manchella, S.
    Parkinson, E. K.
    Prime, S. S.
    [J]. CARCINOGENESIS, 2017, 38 (01) : 76 - 85
  • [6] The hallmarks of cancer A long non-coding RNA point of view
    Gutschner, Tony
    Diederichs, Sven
    [J]. RNA BIOLOGY, 2012, 9 (06) : 703 - 719
  • [7] Long non-coding RNA NKILA inhibits migration and invasion of tongue squamous cell carcinoma cells via suppressing epithelial-mesenchymal transition
    Huang, Wei
    Cui, Xiuying
    Chen, Jianing
    Feng, Yuhuan
    Song, Erwei
    Li, Jinsong
    Liu, Yujie
    [J]. ONCOTARGET, 2016, 7 (38) : 62520 - 62532
  • [8] The lncRNA MIR4435-2HG is upregulated in hepatocellular carcinoma and promotes cancer cell proliferation by upregulating miRNA-487a
    Kong, Qinglei
    Liang, Caiqian
    Jin, Yi
    Pan, Yuhang
    Tong, Dayue
    Kong, Qingcong
    Zhou, Jing
    [J]. CELLULAR & MOLECULAR BIOLOGY LETTERS, 2019, 24 (1)
  • [9] Oral cancer: Etiology and risk factors: A review
    Kumar, Malay
    Nanavati, Ronak
    Modi, Tapan G.
    Dobariya, Chintan
    [J]. JOURNAL OF CANCER RESEARCH AND THERAPEUTICS, 2016, 12 (02) : 458 - 463
  • [10] TGF-β-RI Kinase Inhibitor SD-208 Reduces the Development and Progression of Melanoma Bone Metastases
    Mohammad, Khalid S.
    Javelaud, Delphine
    Fournier, Pierrick G. J.
    Niewolna, Maria
    McKenna, C. Ryan
    Peng, Xiang H.
    Duong, Vu
    Dunn, Lauren K.
    Mauviel, Alain
    Guise, Theresa A.
    [J]. CANCER RESEARCH, 2011, 71 (01) : 175 - 184
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