Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes

被引：1

作者：

He, Yanfeng ^{[1
]}

Sato, Shigeo ^{[1
]}

Tomomori-Sato, Chieri ^{[1
]}

Chen, Shiyuan ^{[1
]}

Goode, Zach H. ^{[1
]}

Conaway, Joan W. ^{[1
,2
,3
]}

Conaway, Ronald C. ^{[1
,2
]}

机构：

[1] Stowers Inst Med Res, Kansas City, MO 64110 USA

[2] Kansas Univ, Med Ctr, Dept Biochem & Mol Biol, Kansas City, KS 66160 USA

[3] UT Southwestern Med Ctr, Dept Mol Biol, Dallas, TX 75390 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2021年 / 118卷 / 39期

关键词：

RNA polymerase II; transcription; Mediator; enhancer; ENDOPLASMIC-RETICULUM STRESS; POLYMERASE-II TRANSCRIPTION; NF-Y CBF; ELONGATION-FACTOR; MAMMALIAN ELONGIN; FACTOR-SIII; IN-VIVO; COMPLEX; PROTEIN; RECRUITMENT;

D O I：

10.1073/pnas.2108751118

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The bZIP transcription factor ATF6 alpha is a master regulator of endo-plasmic reticulum (ER) stress response genes. In this report, we identify the multifunctional RNA polymerase II transcription factor Elongin as a cofactor for ATF6 alpha-dependent transcription activa-tion. Biochemical studies reveal that Elongin functions at least in part by facilitating ATF6 alpha-dependent loading of Mediator at the promoters and enhancers of ER stress response genes. Depletion of Elongin from cells leads to impaired transcription of ER stress response genes and to defects in the recruitment of Mediator and its CDK8 kinase subunit. Taken together, these findings bring to light a role for Elongin as a loading factor for Mediator during the ER stress response.

引用

页数：10

共 63 条

[1] Structure of the human Mediator-bound transcription preinitiation complex [J].