Quantitative study of mammalian cells by scanning transmission soft X-ray microscopy

被引：3
作者：
Shinohara, K. [1 ]
Ohigashi, T. [2 ]
Tone, S. [3 ,5 ]
Kado, M. [4 ]
Ito, A. [1 ]
机构：
[1] Tokai Univ, Sch Engn, Hiratsuka, Kanagawa 2591292, Japan
[2] Inst Mol Sci, UVSOR Synchrotron, Okazaki, Aichi 4448585, Japan
[3] Kawasaki Med Sch, Dept Biochem, Kurashiki, Okayama 7010192, Japan
[4] QST, Kansai Photon Sci Inst, Kyoto 6190215, Japan
[5] Tokyo Denki Univ, Hatoyama, Saitama 3500394, Japan
来源：
X-RAY MICROSCOPY CONFERENCE 2016 (XRM 2016) | 2017年 / 849卷
关键词：
SPECTROSCOPY; DNA;
D O I：
10.1088/1742-6596/849/1/012003
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Molecular distribution in mammalian cells was studied by soft X-ray scanning transmission microscopy with respect to the quantitative aspect of analysis. NEXAFS profiles at the C, N and O K-absorption edges were combined and used for the analysis. For the estimation of quantity for nucleic acids and proteins, NEXAFS profiles of DNA and bovine serum albumin (BSA) at the N K-absorption edge were applied assuming that those were their representatives. The method has a potential to explore the other molecular components than nucleic acids and proteins.
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页数：4
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