Quantitative study of mammalian cells by scanning transmission soft X-ray microscopy

被引：3

作者：

Shinohara, K. ^{[1
]}

Ohigashi, T. ^{[2
]}

Tone, S. ^{[3
,5
]}

Kado, M. ^{[4
]}

Ito, A. ^{[1
]}

机构：

[1] Tokai Univ, Sch Engn, Hiratsuka, Kanagawa 2591292, Japan

[2] Inst Mol Sci, UVSOR Synchrotron, Okazaki, Aichi 4448585, Japan

[3] Kawasaki Med Sch, Dept Biochem, Kurashiki, Okayama 7010192, Japan

[4] QST, Kansai Photon Sci Inst, Kyoto 6190215, Japan

[5] Tokyo Denki Univ, Hatoyama, Saitama 3500394, Japan

来源：

X-RAY MICROSCOPY CONFERENCE 2016 (XRM 2016) | 2017年 / 849卷

关键词：

SPECTROSCOPY; DNA;

D O I：

10.1088/1742-6596/849/1/012003

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Molecular distribution in mammalian cells was studied by soft X-ray scanning transmission microscopy with respect to the quantitative aspect of analysis. NEXAFS profiles at the C, N and O K-absorption edges were combined and used for the analysis. For the estimation of quantity for nucleic acids and proteins, NEXAFS profiles of DNA and bovine serum albumin (BSA) at the N K-absorption edge were applied assuming that those were their representatives. The method has a potential to explore the other molecular components than nucleic acids and proteins.

引用

页数：4

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