Characterization of macrophages infiltrating peri-implantitis lesions

被引:75
作者
Fretwurst, Tobias [1 ,3 ,5 ]
Garaicoa-Pazmino, Carlos [2 ,5 ]
Nelson, Katja [3 ]
Giannobile, William, V [1 ]
Squarize, Cristiane H. [1 ,5 ]
Larsson, Lena [1 ,4 ]
Castilho, Rogerio M. [1 ,5 ]
机构
[1] Univ Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
[2] Oregon Hlth & Sci Univ, Sch Dent, Dept Periodontol, Portland, OR 97201 USA
[3] Univ Freiburg, Fac Med, Med Ctr, Dept Oral & Maxillofacial Surg, Freiburg, Germany
[4] Univ Gothenburg, Inst Odontol, Dept Periodontol, Gothenburg, Sweden
[5] Univ Michigan Sch, Dept Periodont & Oral Med, Lab Epithelial Biol, Ann Arbor, MI USA
关键词
dental implants; histology; inflammation; macrophages; nitric oxide synthase; Peri-implantitis; periodontitis; 2017 WORLD WORKSHOP; CONSENSUS REPORT; M2; MACROPHAGES; CLASSIFICATION; ACTIVATION; DISEASES; POLARIZATION; EXPRESSION; TISSUE; M1;
D O I
10.1111/clr.13568
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives The mechanisms involved in the initiation and progression of peri-implantitis lesions are poorly understood. It was the aim to determine the content and activation status of macrophages present in human peri-implantitis lesions and compare the current findings with the macrophage polarization associated with periodontitis lesions. Material and Methods A total of 14 patients were studied in this investigation. Seven were soft tissue biopsies from dental implants affected by peri-implantitis that required explantation. Seven biopsies were from chronic periodontal disease. Immunofluorescence stains were performed using biomarkers to identify macrophages (CD68(+)) undergoing M1 polarization (iNOS(+)) and M2 polarization (CD206(+)), along with Hoechst 33,342 to identify DNA content. All samples were stained and photographed, and double-positive cells for CD68 and iNOS or CD68 and CD206 were quantified. Results All peri-implantitis biopsies examined revealed a mixed population of macrophages undergoing M1 polarization and M2 polarization. Further analysis demonstrated the co-expression of iNOS and CD206, which indicates the presence of a heterogenic immune response on peri-implantitis lesions. Macrophage polarization in peri-implantitis lesions presents a distinct pattern than in periodontitis. We observed a significant increase in the population of M1 macrophages on peri-implantitis samples compared to periodontal disease samples. Conclusion Our results demonstrate that peri-implantitis has higher numbers of macrophages displaying a distinct macrophage M1 polarization signature compared to periodontitis lesions. This pattern may explain, in part, the distinct nature of peri-implantitis progression vs. periodontitis in humans.
引用
收藏
页码:274 / 281
页数:8
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