Simultaneous analysis of ketamine and bupivacaine in plasma by high-performance liquid chromatography

被引：48
作者：
Gross, AS
Nicolay, A
Eschalier, A
机构：
[1] CHU, Lab Pharmacol Toxicol, F-63003 Clermont Ferrand 1, France
[2] Grp NPPUA, Fac Med, F-63003 Clermont Ferrand, France
来源：
JOURNAL OF CHROMATOGRAPHY B | 1999年 / 728卷 / 01期
关键词：
ketamine; bupivacaine;
D O I：
10.1016/S0378-4347(99)00097-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A reversed-phase HPLC technique for the simultaneous measurement of both bupivacaine and ketamine in plasma is described. Plasma samples (0.5 mi) were prepared using a rapid and simple back-extraction technique. Resolution of both analytes and the internal standard, desipramine, from medicines coadministered to surgical paediatric patients was obtained using a 5 mu m cyano (CN) (250x4.6 mm) column and a mobile phase comprising methanol-acetonitrile-orthophosphoric acid-0.01 M sodium dihydrogenphosphate (200:80:2:718). Good sensitivity for both analytes was observed using UV detection at a wavelength of 215 nm. The method has been validated according to the criteria established by the Journal of Chromatography B. (C) 1999 Elsevier Science BN. All rights reserved.
引用
收藏
页码：107 / 115
页数：9
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