Molecular diagnosis of mango malformation disease and phylogeny of Fusarium mangiferae

被引：17

作者：

Newman, Zvi ^{[2
]}

Freeman, Stanley ^{[1
]}

Biton, Iris ^{[2
]}

Sa'ada, David ^{[2
]}

Paz, Tali ^{[2
]}

Maymon, Marcel ^{[1
,2
]}

Lavi, Uri

机构：

[1] ARO, Volcani Ctr, Dept Plant Pathol & Weed Res, IL-50250 Bet Dagan, Israel

[2] ARO, Volcani Ctr, Dept Fruit Trees, IL-50250 Bet Dagan, Israel

来源：

PHYTOPARASITICA | 2012年 / 40卷 / 03期

关键词：

Amplified fragment length polymorphism (AFLP); Fungal genetics; Genetic variation; Gibberella fujikuroi species complex (GFSC); Mangifera indica; SUBGLUTINANS; DNA; IDENTIFICATION; OXYSPORUM; PATHOGEN; AFLP; MAP;

D O I：

10.1007/s12600-012-0224-6

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Mango malformation disease (MMD) caused by various Fusaria, including the fungus Fusarium mangiferae, is difficult to diagnose and cannot be controlled effectively. In this study a PCR diagnostic tool was developed for detection of F. mangiferae by generating primers which flank fungus-specific sequences. The assay is suitable for both in-vitro and in-vivo tests, and is capable of detecting 10 pg of the fungal DNA. Relatively high genetic variation was discovered between various isolates of the pathogen based on amplified fragment length polymorphism (AFLP) analyses. In addition, some isolates of F. mangiferae (including the accession numbers FMS-123 and NRRL 25226), were found to be quite distinct from most F. mangiferae accessions. Likewise, representative MMD-associated Fusaria from the Asian and American clades of the Gibberella fujikuroi species complex were further differentiated by AFLP analyses.

引用

页码：287 / 297

页数：11

共 28 条

[1]

Abd-Elsalam A., 2003, J PLANT PATHOL, V85, P103

[2] Two new species of Fusarium section Liseola associated with mango malformation [J].

Britz, H ;

Steenkamp, ET ;

Coutinho, TA ;

Wingfield, BD ;

Marasas, WFO ;

Wingfield, MJ .

MYCOLOGIA, 2002, 94 (04) :722-730

[3]

Buntjer JB, 2001, PHYLTOOLS PHYLOGENET

[4]

FELSENSTEIN J, 1999, PHYLIP PHYLOGENY INF

[5] Use of GUS transformants of Fusarium subglutinans for determining etiology of mango malformation disease [J].

Freeman, S ;

Maimon, M ;

Pinkas, Y .

PHYTOPATHOLOGY, 1999, 89 (06) :456-461

[6] Cultivar identification and genetic map of mango (Mangifera indica) [J].

Kashkush, K ;

Jinggui, F ;

Tomer, E ;

Hillel, J ;

Lavi, U .

EUPHYTICA, 2001, 122 (01) :129-136

[7] RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM GROUPS AND PHYSICAL MAP OF MITOCHONDRIAL-DNA FROM FUSARIUM-OXYSPORUM F SP-NIVEUM [J].

KIM, DH ;

MARTYN, RD ;

MAGILL, CW .

PHYTOPATHOLOGY, 1992, 82 (03) :346-353

[8]

KUMAR J, 1993, ANNU REV PHYTOPATHOL, V31, P217, DOI 10.1146/annurev.py.31.090193.001245

[9] A new Fusarium lineage within the Gibberella fujikuroi species complex is the main causal agent of mango malformation disease in Brazil [J].

Lima, C. S. ;

Pfenning, L. H. ;

Costa, S. S. ;

Campos, M. A. ;

Leslie, J. F. .

PLANT PATHOLOGY, 2009, 58 (01) :33-42

[10] VCG and AFLP analyses identify the same groups in the causal agents of mango malformation in Brazil [J].

Lima, Cristiano S. ;

Monteiro, Jean H. A. ;

Crespo, Natalia C. ;

Costa, Sarah S. ;

Leslie, John F. ;

Pfenning, Ludwig H. .

EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2009, 123 (01) :17-26

← 1 2 3 →