Surface display of functional fibronectin-binding domains on Staphylococcus carnosus

被引：22
|
作者：
Liljeqvist, S
Cano, F
Nguyen, TN
Uhlén, M
Robert, A
Ståhl, S
机构：
[1] Royal Inst Technol, Dept Biotechnol, S-10044 Stockholm, Sweden
[2] Ctr Immunol Pierre Fabre, F-74164 St Julien En Genevois, France
来源：
FEBS LETTERS | 1999年 / 446卷 / 2-3期
关键词：
surface display; fibronectin binding; staphylococcal protein A; albumin binding protein; mucosal immunization; Staphylococcus carnosus;
D O I：
10.1016/S0014-5793(99)00232-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The surface expression in Staphylococcus carnosus of three different fibronectin binding domains (FNBDs), derived from fibronectin binding proteins of Streptococcus dysgalactiae and staphylococcus aureus, has been investigated. Surface localization of the chimeric proteins containing the FNBDs was demonstrated. All three surface-displayed FNBDs were demonstrated to bind fibronectin in whole-cell enzyme-linked binding assays. Furthermore, for one of the constructs, intranasal immunizations with the recombinant bacteria resulted in improved antibody responses to a model immunogen present within the chimeric surface proteins. The implications of the results for the design of live bacterial vaccine delivery systems are discussed. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码：299 / 304
页数：6
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