Reduction in circulating bile acid and restricted diffusion across the intestinal epithelium are associated with a decrease in blood cholesterol in the presence of oat β-glucan

被引:65
作者
Gunness, Purnima [1 ]
Michiels, Joris [2 ]
Vanhaecke, Lynn [3 ]
De Smet, Stefaan [4 ]
Kravchuk, Olena [5 ]
Van de Meene, Allison [6 ]
Gidley, Michael J. [1 ]
机构
[1] Univ Queensland, Australian Res Council, Ctr Excellence Plant Cell Walls, Ctr Nutr & Food Sci,Queensland Alliance Agr & Foo, Hartley Teakle Bldg 83, Brisbane, Qld 4072, Australia
[2] Univ Ghent, Dept Appl Biosci, Fac Biosci Engn, Ghent, Belgium
[3] Univ Ghent, Fac Biosci Engn, Dept Anim Prod, Melle, Belgium
[4] Univ Ghent, Dept Vet Publ Hlth & Food Safety, Fac Vet Med, Merelbeke, Belgium
[5] Univ Adelaide, Sch Agr Food & Wine, Biometry Hub, Glen Osmond, SA, Australia
[6] Univ Melbourne, Australian Res Council, Ctr Excellence Plant Cell Walls, Sch Biosci Bot, Parkville, Vic, Australia
基金
澳大利亚研究理事会;
关键词
Ussing chamber; microscopy; nonstarch polysaccharides; deoxycholic acid; soluble dietary fiber; DIETARY FIBER; EXCRETION; MODEL; PIG; TRIGLYCERIDES; PERMEABILITY; ARABINOXYLAN; MODULATION; MECHANISMS; DIGESTION;
D O I
10.1096/fj.201600465R
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although oat (1,3: 1,4)-beta-glucan (BG) has been shown to decrease blood cholesterol in intervention trials, the detailed mechanism is not yet defined, but restricted reabsorption of bile acids (BAs) has been hypothesized. Using pigs as a model for humans we demonstrated that, compared to the control, BG added to the diet for 26 d caused decreases of 24% in blood total BAs (TBAs), 34% in total cholesterol (TC), and 57% in LDL cholesterol (LDL-C) (P < 0.01); decreases of 20% TBA in the midjejunumand terminal ileum(P < 0.01); increases of 80% in cecal total neutral sterols (TNSs) including cholesterol (P < 0.01); a 50% reduction in BA active transport across ex vivo ileum after 40 min (P < 0.001); and 32% decrease in jejunal microvillus heights with apparent increased goblet cell activity. The results suggest that BG not only physically hinders the active reabsorption of BAs and uptake of cholesterol, but also changes the BAs profile with lower circulating levels without excess excretion in the feces, thus resulting in reduced blood TC and LDL-C. Fermentation of sterols reaching the colon enhanced production of therapeutic ursodeoxycholic acid, suppressed toxic lithocholic acid, and decreased the possibility of cholesterol absorption by transforming the latter into coprostanol, a nonabsorbable NS.
引用
收藏
页码:4227 / 4238
页数:12
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