Intravenously delivered neural stem cells migrate into ischemic brain, differentiate and improve functional recovery after transient ischemic stroke in adult rats

被引：1
作者：
Cheng, Yi [1 ]
Zhang, Jinjing [1 ]
Deng, Liancheng [1 ,2 ]
Johnson, Noah R. [3 ,4 ]
Yu, Xichong [1 ]
Zhang, Ning [2 ]
Lou, Tianzheng [2 ]
Zhang, Yi [1 ]
Wei, Xiaojie [5 ]
Chen, Zaifeng [5 ]
He, Songbin [6 ]
Li, Xiaokun [1 ]
Xiao, Jian [1 ]
机构：
[1] Wenzhou Med Univ, Sch Pharmaceut Sci, Inst Mol Pharmacol, Wenzhou 325035, Peoples R China
[2] Wenzhou Med Univ, Lishui Peoples Hosp, Translat Med Res Ctr, Lishui 323000, Zhejiang, Peoples R China
[3] Univ Pittsburgh, Dept Bioengn, Pittsburgh, PA 15219 USA
[4] Univ Pittsburgh, McGowan Inst Regenerat Med, Pittsburgh, PA 15219 USA
[5] Wenzhou Med Univ, Cixi Peoples Hosp, Dept Neurosurg, Ningbo 315300, Zhejiang, Peoples R China
[6] Wenzhou Med Univ, Zhoushan Hosp, Dept Neurol, Zhoushan 316000, Zhejiang, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2015年 / 8卷 / 03期
关键词：
Ischemic stroke; cell transplantation; neural stem cells; intravenous administration; MARROW STROMAL CELLS; CEREBRAL-ISCHEMIA; INFARCT VOLUME; TRANSPLANTATION; MODEL; INJURY; REPLACEMENT; PRECURSORS; DEFICITS; THERAPY;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Stem cell transplantation may provide an alternative therapy to promote functional recovery after various neurological disorders including cerebral infarct. Due to the minimal immunogenicity and neuronal differentiation potential of neural stem cells (NSCs), we tested whether intravenous administration of mice-derived C17.2 NSCs could improve neurological function deficit and cerebral infarction volume after ischemic stroke in rats. Additionally, we evaluated the survival, migration, proliferation, and differentiation capacity of transplanted NSCs in the rat brain. Intravenous infusion of NSCs after middle cerebral artery occlusion (MCAO) showed better performance in neurobiological severity scores after MCAO compared to control. However, the volume of cerebral infarction was not different at 7 days after MCAO compared with control. Transplanted NSCs were detected in the ischemic region but not in the contralateral hemisphere. NSCs differentiated into neurons or astrocytes after MCAO. These data suggest that intravenously transplanted NSCs can migrate, proliferate, and differentiate into neurons and astrocytes in the rat brain with focal ischemia and improve functional recovery.
引用
收藏
页码：2928 / 2936
页数：9
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