Use of a new Trichoderma harzianum strain isolated from the Amazon rainforest with pretreated sugar cane bagasse for on-site cellulase production

被引：102

作者：

Delabona, Priscila da Silva ^{[1
]}

Farinas, Cristiane Sanchez ^{[2
]}

da Silva, Mateus Ribeiro ^{[1
]}

Azzoni, Sindelia Freitas ^{[1
]}

da Cruz Pradella, Jose Geraldo ^{[1
]}

机构：

[1] Brazilian Bioethanol Sci & Technol Lab CTBE, Ind Program, BR-13083970 Campinas, SP, Brazil

[2] Embrapa Instrumentat, BR-13560970 Sao Paulo, Brazil

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 107卷

关键词：

On-site enzyme production; Trichoderma harzianum; Cellulase; Submerged fermentation; Sugar cane bagasse; BETA-GLUCOSIDASE; ENZYMES;

D O I：

10.1016/j.biortech.2011.12.048

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The on-site production of cellulases is an important strategy for the development of sustainable second-generation ethanol production processes. This study concerns the use of a specific cellulolytic enzyme complex for hydrolysis of pretreated sugar cane bagasse. Glycosyl hydrolases (FPase, xylanase, and beta-glucosidase) were produced using a new strain of Trichoderma harzianum, isolated from the Amazon rainforest and cultivated under different conditions. The influence of the carbon source was first investigated using shake-flask cultures. Selected carbon sources were then further studied under different pH conditions using a stirred tank bioreactor. Enzymatic activities up to 121 FPU/g, 8000IU/g, and 1730IU/g of delignified steam-exploded bagasse + sucrose were achieved for cellulase, xylanase and beta-glucosidase, respectively. This enzymatic complex was used to hydrolyze pretreated sugar cane bagasse. A comparative evaluation, using an enzymatic extract from Trichoderma reesei RUTC30, indicated similar performance of the T. harzianum enzyme complex, being a potential candidate for on-site production of enzymes. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：517 / 521

页数：5

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