Hydrogen/deuterium exchange behavior in tetragonal hen egg-white lysozyme crystals affected by solution state

被引：7
作者：
Kita, Akiko [1 ]
Morimoto, Yukio [1 ]
机构：
[1] Kyoto Univ, Inst Integrated Radiat & Nucl Sci, Kumatori, Osaka 5900494, Japan
来源：
JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2020年 / 53卷
关键词：
hen egg-white lysozyme; H/D exchange; crystal structure; X-ray/neutron joint refinement; JOINT X-RAY; HYDROGEN-EXCHANGE; NEUTRON-DIFFRACTION; PROTEIN DYNAMICS; PROGRAM; REFINEMENT;
D O I：
10.1107/S1600576720005488
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Neutron diffraction studies of hydrogen/deuterium-exchanged hen egg-white lysozyme were performed by a joint X-ray and neutron refinement to elucidate the hydrogen/deuterium exchange behavior. Large crystals for neutron work, consisting of molecules that were exchanged before crystallization, were obtained by repeatedly adding protein solution to the crystal batch using deuterated precipitant reagent. There are differences in hydrogen/deuterium exchange behavior compared with previous crystallographic or NMR studies, which could be due to intermolecular interactions in the crystal or to different lengths of exchange period.
引用
收藏
页码：837 / 840
页数：4
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共 30 条
  • [1] Towards automated crystallographic structure refinement with phenix.refine
    Afonine, Pavel V.
    Grosse-Kunstleve, Ralf W.
    Echols, Nathaniel
    Headd, Jeffrey J.
    Moriarty, Nigel W.
    Mustyakimov, Marat
    Terwilliger, Thomas C.
    Urzhumtsev, Alexandre
    Zwart, Peter H.
    Adams, Paul D.
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2012, 68 : 352 - 367
  • [2] Joint X-ray and neutron refinement with phenix.refine
    Afonine, Pavel V.
    Mustyakimov, Marat
    Grosse-Kunstleve, Ralf W.
    Moriarty, Nigel W.
    Langan, Paul
    Adams, Paul D.
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 : 1153 - 1163
  • [3] LSCALE -: the new normalization, scaling and absorption correction program in the Daresbury Laue software suite
    Arzt, S
    Campbell, JW
    Harding, MM
    Hao, Q
    Helliwell, JR
    [J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1999, 32 : 554 - 562
  • [4] Neutron diffraction studies of Escherichia coli dihydrofolate reductase complexed with methotrexate
    Bennett, Brad
    Langan, Paul
    Coates, Leighton
    Mustyakimov, Marat
    Schoenborn, Benno
    Howell, Elizabeth E.
    Dealwis, Chris
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (49) : 18493 - 18498
  • [5] EXCHANGE OF INDIVIDUAL HYDROGENS FOR A PROTEIN IN A CRYSTAL AND IN SOLUTION
    BENTLEY, GA
    DELEPIERRE, M
    DOBSON, CM
    WEDIN, RE
    MASON, SA
    POULSEN, FM
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1983, 170 (01) : 243 - 247
  • [6] Quasi-Laue neu iron-diffraction study of the water arrangement in crystals of triclinic hen egg-white lysozyme
    Bon, C
    Lehmann, MS
    Wilkinson, C
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1999, 55 : 978 - 987
  • [7] LAUEGEN version 6.0 and INTLDM
    Campbell, JW
    Hao, Q
    Harding, MM
    Nguti, ND
    Wilkinson, C
    [J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1998, 31 : 496 - 502
  • [8] LAUEGEN, AN X-WINDOWS-BASED PROGRAM FOR THE PROCESSING OF LAUE X-RAY-DIFFRACTION DATA
    CAMPBELL, JW
    [J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1995, 28 : 228 - 236
  • [9] MolProbity: all-atom structure validation for macromolecular crystallography
    Chen, Vincent B.
    Arendall, W. Bryan, III
    Headd, Jeffrey J.
    Keedy, Daniel A.
    Immormino, Robert M.
    Kapral, Gary J.
    Murray, Laura W.
    Richardson, Jane S.
    Richardson, David C.
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 : 12 - 21
  • [10] ELECTROSTATIC EFFECTS AND HYDROGEN-EXCHANGE BEHAVIOR IN PROTEINS - THE PH-DEPENDENCE OF EXCHANGE-RATES IN LYSOZYME
    DELEPIERRE, M
    DOBSON, CM
    KARPLUS, M
    POULSEN, FM
    STATES, DJ
    WEDIN, RE
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1987, 197 (01) : 111 - 122
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