SNPs in the Toni receptor of Litopenaeus vannamei are associated with immune response

被引:21
作者
Yao, Defu [1 ,2 ]
Su, Huimin [1 ,2 ]
Zhu, Jinghua [1 ,2 ]
Zhao, Xianliang [3 ]
Aweya, Jude Juventus [1 ,2 ]
Wang, Fan [1 ,2 ]
Zhong, Mingqi [1 ,2 ]
Zhang, Yueling [1 ,2 ]
机构
[1] Shantou Univ, Dept Biol, Shantou 515063, Peoples R China
[2] Shantou Univ, Guangdong Prov Key Lab Marine Biotechnol, Shantou 515063, Peoples R China
[3] Henan Normal Univ, Coll Fisheries, Xinxiang 453007, Peoples R China
基金
中国国家自然科学基金;
关键词
Shrimp; Toll1; receptor; SNPs; Pathogen infections; Resistance; SINGLE NUCLEOTIDE POLYMORPHISMS; MESSENGER-RNA EXPRESSION; GENOME-WIDE ASSOCIATION; TOLL-LIKE RECEPTORS; AEROMONAS-HYDROPHILA; PATHOGEN RECOGNITION; GRASS CARP; GENE; RESISTANCE; SUSCEPTIBILITY/RESISTANCE;
D O I
10.1016/j.fsi.2017.11.018
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Tolls and Toll-like receptors (TLRs) are important regulators in the innate immune system and their genetic variations usually affect the host's susceptibility/resistance to pathogen infections. In this study, we report on the single nucleotide polymorphisms (SNPs) of Toll1 in Litopenaeus vannamei (LvToll1) and how this is associated with immune response. PCR-DGGE analysis revealed genetic polymorphisms in LvToll1 at both the genomic DNA (gDNA) and cDNA levels. Using high-throughput sequencing, 223 SNPs were identified at the gDNA level, of which 145 were non-synonymous SNP (nsSNP), with 3 nsSNPs having frequency over 1%. On the other hand, 60 SNPs were identified at the cDNA level including 38 nsSNPs and 4 nsSNPs with frequency over 1%. Upon challenging shrimps with Streptococcus iniae, Vibrio parahaemolyticus and white spot syndrome virus (WSSV), LvToll1 was shown to generate 6, 4 and 4 novel bands, respectively when analyzed with PCR-DGGE. Sequencing analysis of these bands showed that they contained 6, 4 and 2 nsSNPs, respectively. Moreover, the nsSNP C1526T was detected in S. iniae-resistant but not in susceptible shrimps. Most significantly, the C1526T mutation could shorten the alpha-helix of the LRR domain and was predicted to affect the function of LvToll1, indicating that SNP C1526T might be associated with shrimp's resistance to pathogen infections. In sum, our findings here reveal that the genetic polymorphisms of Toll receptor are linked with the immune response to pathogen infections in L. vannamei.
引用
收藏
页码:410 / 417
页数:8
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