Sequence-specific binding property of Arabidopsis thaliana telomeric DNA binding protein 1 (AtTBP1)

被引：62

作者：

Hwang, MG ^{[1
]}

Chung, IK ^{[1
]}

Kang, BG ^{[1
]}

Cho, MH ^{[1
]}

机构：

[1] Yonsei Univ, Dept Biol, Seoul 120749, South Korea

来源：

FEBS LETTERS | 2001年 / 503卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

telomere-binding protein; telomere; electrophoresis mobility shift assay; Arabidopsis thaliana;

D O I：

10.1016/S0014-5793(01)02685-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arahidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a single Myb telomeric DNA binding domain at the C-terminus and an extensive homology with other known telomere-binding proteins. The isolated C-terminus of AtTBP1 is capable of sequence-specific DNA binding to plant duplex telomeric DNA. These results suggest that AtTBP1 may play important roles in plant telomere function in vivo. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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页码：35 / 40

页数：6

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