Microwave-assisted solid-phase peptide synthesis of neurosecretory protein GL composed of 80 amino acid residues

被引：23

作者：

Masuda, Keiko ^{[1
]}

Ooyama, Haruka ^{[1
]}

Shikano, Kenshiro ^{[1
]}

Kondo, Kunihiro ^{[1
]}

Furumitsu, Megumi ^{[1
]}

Iwakoshi-Ukena, Eiko ^{[1
]}

Ukena, Kazuyoshi ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Integrated Arts & Sci, Sect Behav Sci, Higashihiroshima 7398521, Japan

来源：

JOURNAL OF PEPTIDE SCIENCE | 2015年 / 21卷 / 06期

关键词：

small protein; NPGL; microwave; solid-phase peptide synthesis; disulfide bond formation; COMPLEX PEPTIDES; REAGENTS;

D O I：

10.1002/psc.2756

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recently identified a novel cDNA encoding a small secretory protein of 80 amino acid residues, termed neurosecretory protein GL (NPGL), from the chicken hypothalamus. Homologs of NPGL have been reported to be present in mammals, such as human and rat. NPGL is amidated at its C-terminus, contains an intramolecular disulfide bond, and is hydrophobic in nature. In this study, we have optimized the synthesis of the entire 80-amino acid peptide sequence of rat NPGL by microwave-assisted solid-phase peptide synthesis. NPGL was obtained with a 10% yield when the coupling reactions were performed using 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium-3-oxid hexafluorophosphate (HATU) at 50 degrees C for 5min, and Fmoc deprotections were performed using 40% piperidine containing 0.1M HOBt. Furthermore, the disulfide bond of NPGL was formed with 20% yield with the use of glutathione-containing redox buffer and 50% acetonitrile. Copyright (c) 2015 European Peptide Society and John Wiley & Sons, Ltd.

引用

页码：454 / 460

页数：7

共 50 条

[41] Amino-Li-Resin-A Fiber Polyacrylamide Resin for Solid-Phase Peptide Synthesis [J].

Akintayo, Damilola C. ;

de la Torre, Beatriz G. ;

Li, Yongfu ;

Albericio, Fernando .

POLYMERS, 2022, 14 (05)

[42] Synthesis of Peptides Histamine H2 Receptors in Solid-Phase Assisted by Microwave [J].

Reynoso-Soto, Edgar A. ;

Rivero, Ignacio A. .

JOURNAL OF THE MEXICAN CHEMICAL SOCIETY, 2010, 54 (03) :160-163

[43] Microwave-assisted reaction of peptide formation by amino acid with phosphate: Exploration of the most possible channels for the origin of life [J].

Hu Rong ;

Tian Jinping ;

Yin Yingwu .

SCIENCE IN CHINA SERIES B-CHEMISTRY, 2006, 49 (04) :351-356

[44] Microwave-assisted reaction of peptide formation by amino acid with phosphate:Exploration of the most possible channels for the origin of life [J].

HU Rong TIAN Jinping YIN Yingwu College of Chemistry Environment Protection Engineering Southwest University for Nationalities Chengdu China THUNIS Insight Co Ltd Beijing China Key Laboratory of Bioorganic Phosphorus Chemistry Chemical Biology Ministry of Education Department of Chemistry Tsinghua University Beijing China .

Science in China(Series B:Chemistry), 2006, (04) :351-356

[45] Microwave-assisted reaction of peptide formation by amino acid with phosphate: Exploration of the most possible channels for the origin of life [J].