NAADP receptors are present and functional in the heart

被引:56
作者
Bak, J
Billington, RA
Timar, G
Dutton, AC
Genazzani, AA
机构
[1] Univ Cambridge, Dept Pharmacol, Cambridge CB2 1QJ, England
[2] Semmelweis Univ, Dept Med Biochem, H-1085 Budapest, Hungary
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1016/S0960-9822(01)00269-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Alongside the well-studied inositol 1,4,5 trisphosphate and ryanodine receptors, evidence is gathering that a new intracellular release mechanism, gated by the pyridine nucleotide nicotinic acid adenine dinucleotide phosphate (NAADP), is present in numerous organisms, ranging from plant to mammalian cells (reviewed in [1]), Most cells have been shown to express at least two Ca2+-release mechanisms controlled by different messengers, and this can lead to redundancy, convergence, or divergence of responses. One exception appears to be muscle and heart contractile tissues. Here, it is thought that the dominant intracellular channel is the ryanodine receptor, while IF, receptors are poorly expressed and their role appears to be negligible, We now report that NAADP receptors are functional and abundant in cardiac microsomes. NAADP binds specifically and with high affinity (130 pM and 4 nM) to two sites on cardiac microsomes and releases Ca2+ with an apparent EC50 of 323 +/- 14 nM, Furthermore, binding experiments show that this receptor displays both positive and negative cooperativity, a peculiarity unique among intracellular Ca2+ channels. Therefore, we show that the heart possesses multiple mechanisms to Increase the complexity of Ca2+ signaling and that NAADP may be integral in the functioning of this organ. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:987 / 990
页数:4
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