The B-cell maturation factor Blimp-1 specifies vertebrate slow-twitch muscle fiber identity in response to Hedgehog signaling

被引:152
作者
Baxendale, S
Davison, C
Muxworthy, C
Wolff, C
Ingham, PW
Roy, S
机构
[1] Univ Sheffield, Sch Med & Biomed Sci, Ctr Dev Genet, MRC Intecellular Signaling Grp, Sheffield S10 2TN, S Yorkshire, England
[2] Inst Mol & Cell Biol, Singapore 117609, Singapore
基金
英国医学研究理事会;
关键词
D O I
10.1038/ng1280
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Vertebrate skeletal muscles comprise distinct fiber types that differ in their morphology, contractile function, mitochondrial content and metabolic properties. Recent studies identified the transcriptional coactivator PGC-1 as a key mediator of the physiological stimuli that modulate fiber-type plasticity in postembryonic development(1). Although myoblasts become fated to differentiate into distinct kinds of fibers early in development, the identities of regulatory proteins that determine embryonic fiber-type specification are still obscure. Here we show that the gene u-boot (ubo), a mutation in which disrupts the induction of embryonic slow-twitch fibers(2), encodes the zebrafish homolog of Blimp-1, a SET domain containing transcription factor that promotes the terminal differentiation of B lymphocytes in mammals(3). Expression of ubo is induced by Hedgehog (Hh) signaling in prospective slow muscle precursors, and its activity alone is sufficient to direct slow-twitch fiber specific development by naive myoblasts. Our data provide the first molecular insight into the mechanism by which a specific group of muscle precursors is driven along a distinct pathway of fiber-type differentiation in response to positional cues in the vertebrate embryo.
引用
收藏
页码:88 / 93
页数:6
相关论文
共 29 条
[1]  
Barresi MJF, 2000, DEVELOPMENT, V127, P2189
[2]   Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog [J].
Blagden, CS ;
Currie, PD ;
Ingham, PW ;
Hughes, SM .
GENES & DEVELOPMENT, 1997, 11 (17) :2163-2175
[3]  
Borycki AG, 1998, DEVELOPMENT, V125, P777
[4]   The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development [J].
Chang, DH ;
Calame, KL .
MECHANISMS OF DEVELOPMENT, 2002, 117 (1-2) :305-309
[5]   Hedgehog signalling is required for maintenance of myf5 and myoD expression and timely terminal differentiation in zebrafish adaxial myogenesis [J].
Coutelle, O ;
Blagden, CS ;
Hampson, R ;
Halai, C ;
Rigby, PWJ ;
Hughes, SM .
DEVELOPMENTAL BIOLOGY, 2001, 236 (01) :136-150
[6]   Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish [J].
Currie, PD ;
Ingham, PW .
NATURE, 1996, 382 (6590) :452-455
[7]   The zinc finger gene Xblimp1 controls anterior endomesodermal cell fate in Spemann's organizer [J].
de Souza, FSJ ;
Gawantka, V ;
Gómez, AP ;
Delius, H ;
Ang, SL ;
Niehrs, C .
EMBO JOURNAL, 1999, 18 (21) :6062-6072
[8]  
Devoto SH, 1996, DEVELOPMENT, V122, P3371
[9]   Inhibition of zebrafish fgf8 Pre-mRNA splicing with morpholino oligos:: A quantifiable method for gene knockdown [J].
Draper, BW ;
Morcos, PA ;
Kimmel, CB .
GENESIS, 2001, 30 (03) :154-156
[10]   Positive and negative regulation of muscle cell identity by members of the hedgehog and TGF-beta gene families [J].
Du, SJ ;
Devoto, SH ;
Westerfield, M ;
Moon, RT .
JOURNAL OF CELL BIOLOGY, 1997, 139 (01) :145-156