A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite

被引：50

作者：

Xu, X. X. ^{[2
]}

Zhang, J. X. ^{[2
]}

Guo, F. ^{[2
]}

Zheng, W. ^{[2
]}

Zhou, H. M. ^{[2
]}

Wang, B. L. ^{[2
]}

Zheng, Y. F. ^{[1
,2
]}

Wang, Y. B. ^{[1
,3
]}

Cheng, Y. ^{[3
]}

Lou, X. ^{[2
,4
]}

Jang, B. Z. ^{[5
]}

机构：

[1] Peking Univ, Dept Adv Mat & Nanotechnol, Coll Engn, Beijing 100871, Peoples R China

[2] Harbin Engn Univ, Ctr Biomed Mat & Engn, Harbin 150001, Peoples R China

[3] Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China

[4] Curtin Univ Technol, Dept Chem Engn, Bentley, WA 9102, Australia

[5] Wright State Univ, Coll Engn & Comp Sci, Dayton, OH 45435 USA

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2011年 / 84卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Biosensor; Hemoglobin; Nanographene platelets; Direct electron transfer; Electrocatalysis; DIRECT ELECTRON-TRANSFER; DIRECT ELECTROCHEMISTRY; GRAPHENE NANOSHEETS; GLUCOSE-OXIDASE; CYTOCHROME-C; HEMOGLOBIN; ELECTROCATALYSIS; NANOCOMPOSITES; NANOPARTICLES; FABRICATION;

D O I：

10.1016/j.colsurfb.2011.01.037

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Gamma*) and apparent heterogeneous electron transfer rate constant (k(s)) were calculated to be (1.60 +/- 0.17) x 10(-10) mol cm(-2) and 48.51 s(-1), respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H2O2. The biosensor of H2O2 exhibited a linear response to H2O2 in the range of 10-150 mu M and a detection limit of 8.24 mu M (S/N = 3) was obtained. The apparent Michaelis-Menten constant (K-m(app)) was 45.35 mu M. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：427 / 432

页数：6

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