Down-regulation of β1,4GalT V at protein level contributes to arsenic trioxide-induced glioma cell apoptosis

被引：22

作者：

Wei, Yuanyan ^{[1
]}

Liu, Dan ^{[1
]}

Ge, Yuqing ^{[1
]}

Zhou, Fengbiao ^{[1
]}

Xu, Jiejie ^{[1
]}

Chen, Hong ^{[1
]}

Yun, Xiaojing ^{[1
]}

Gu, Jianxin ^{[1
]}

Jiang, Jianhai ^{[1
]}

机构：

[1] Fudan Univ, Shanghai Med Coll, Minist Publ Hlth & Gene Res Ctr, Key Lab Glycoconjuates Res, Shanghai 200032, Peoples R China

来源：

CANCER LETTERS | 2008年 / 267卷 / 01期

关键词：

arsenic trioxide; apoptosis; beta 1,4-galactosyltransferase V; N-glycan;

D O I：

10.1016/j.canlet.2008.03.019

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Arsenic trioxide (As2O3) has considerable efficacy in treating solid tumors with induction of apoptosis with largely unknown mechanisms. Posttranslational processing of proteins by glycosylation could have multiple regulating roles in the process of apoptosis. Here, we found that the expression of beta 1,6-linked GlcNAc-bearing N-glycans on cell surface protein was gradually decreased after induction of apoptosis by As2O3-treatment. And, As2O3 significantly decreased the protein expression level of beta 1,4GalT V, which effectively galactosylates the beta 1,6-GlcNAc branch of N-glycans and functions as a positive regulator in glioma development. Furthermore, interfering with the expression of beta 1,4GalT V in human glioma cell markedly promoted As2O3-induced cell apoptosis and beta 1,4GaIT V overexpression significantly reduced As2O3-induced glioma cell apoptosis. Taken together, our results suggested that down-regulation of PI,4GaIT V expression plays an important role in As2O3-induced apoptosis, providing a new mechanism of As2O3-induced cell apoptosis and indicating that inhibitors of 01,4GaIT V may enhance the therapeutic efficiency of As2O3 for malignant glioma. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：96 / 105

页数：10

共 44 条

[1] Arsenic trioxide induces gallbladder carcinoma cell apoptosis via downregulation of Bcl-2 [J].

Ai, Zhilong ;

Lu, Weiqi ;

Qin, Xinyu .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 348 (03) :1075-1081

[2] The effects of arsenic trioxide (As2O3) on human megakaryocytic leukemia cell lines -: With a comparison of its effects on other cell lineages [J].

Alemany, M ;

Levin, J .

LEUKEMIA & LYMPHOMA, 2000, 38 (1-2) :153-+

[3] Introduction: The history of arsenic trioxide in cancer therapy [J].

Antman, KH .

ONCOLOGIST, 2001, 6 :1-2

[4] COMPARISON OF N-ACETYLGLUCOSAMINYLTRANSFERASE-V ACTIVITIES IN ROUS SARCOMA-TRANSFORMED BABY HAMSTER-KIDNEY (RS-BHK) AND BHK CELLS [J].

ARANGO, J ;

PIERCE, M .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1988, 37 (02) :225-231

[5] STUDY OF O-GLYCAN SIALYLATION IN C6 CULTURED GLIOMA-CELLS - EVIDENCE FOR POSTTRANSLATIONAL REGULATION OF A BETA-GALACTOSIDE-ALPHA-2,3 SIALYLTRANSFERASE ACTIVITY BY N-GLYCOSYLATION [J].

BROQUET, P ;

GEORGE, P ;

GEOFFROY, J ;

REBOUL, P ;

LOUISOT, P .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 178 (03) :1437-1443

[6] Down-regulation of the expression of β1,4-galactosyltransferase V promotes integrin β1 maturation [J].

Chen, XN ;

Jiang, JH ;

Yang, JW ;

Chen, C ;

Sun, MY ;

Wei, YY ;

Guang, XY ;

Gu, JX .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 343 (03) :910-916

[7] Metastases: the glycan connection [J].

Couldrey, C ;

Green, JE .

BREAST CANCER RESEARCH, 2000, 2 (05) :321-323

[8] Coordination of intrinsic, extrinsic, and endoplasmic reticulum-mediated apoptosis by imatinib mesylate combined with arsenic trioxide in chronic myeloid leukemia [J].

Du, YZ ;

Wang, KK ;

Fang, H ;

Li, JM ;

Xiao, D ;

Zheng, PZ ;

Chen, YL ;

Fan, HY ;

Pan, XL ;

Zhao, CJ ;

Zhang, QH ;

Imbeaud, S ;

Graudens, E ;

Eveno, E ;

Auffray, C ;

Chen, SJ ;

Chen, Z ;

Zhang, J .

BLOOD, 2006, 107 (04) :1582-1590

[9] Human Kupffer cell recognition and phagocytosis of apoptotic peripheral blood lymphocytes [J].

Falasca, L ;

Bergamini, A ;

Serafino, A ;

Balabaud, C ;

Dini, L .

EXPERIMENTAL CELL RESEARCH, 1996, 224 (01) :152-162

[10] Apoptosis in liver damage produced by tunicamycin [J].

Finnie, JW ;

Read, SH ;

Swift, JG .

AUSTRALIAN VETERINARY JOURNAL, 2004, 82 (1-2) :87-90

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