Kinetic plot based comparison of the efficiency and peak capacity of high-performance liquid chromatography columns: Theoretical background and selected examples

被引：55
作者：
Broeckhoven, Ken [1 ]
Cabooter, Deirdre [1 ]
Eeltink, Sebastiaan [1 ]
Desmet, Gert [1 ]
机构：
[1] Vrije Univ Brussel, Dept Chem Engn, B-1050 Brussels, Belgium
来源：
JOURNAL OF CHROMATOGRAPHY A | 2012年 / 1228卷
关键词：
Kinetic plot; Superficially porous; Gradient elution; High temperature; High pressure; Monolithic columns; Kinetic performance; SUB-2; MU-M; PARTICLE-SIZE DISTRIBUTION; SILICA CAPILLARY COLUMNS; ULTRA-HIGH-PRESSURE; GRADIENT ELUTION; HIGH-TEMPERATURE; LC SUPPORTS; SEPARATIONS; SYSTEMS; SPEED;
D O I：
10.1016/j.chroma.2011.08.003
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The present contribution reviews the foundations of the kinetic-plot method for the direct comparison of the kinetic performance of different chromatographic support and operating modes. The method directly uses experimental data collected for a specific sample and operating condition of one's interest, and is applicable both under isocratic- and gradient-elution conditions. Experimental proof is provided for the strong relation between the kinetic performance of a given support under isocratic and gradient conditions: a material offering superior kinetic performances under isocratic conditions will remain superior under gradient conditions and vice versa provided the comparison occurs under unbiased conditions. In addition, a review is made of the recent literature using the kinetic-plot method to compare and assess the kinetic performance of high performance HPLC columns and their operation mode. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：20 / 30
页数：11
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