Determination of the spatial structure of glutathione by residual dipolar coupling analysis

被引：16

作者：

Klochkov, AV ^{[1
]}

Khairutdinov, BI ^{[1
]}

Tagirov, MS ^{[1
]}

Klochkov, VV ^{[1
]}

机构：

[1] Kazan VI Lenin State Univ, Dept Phys, Kazan 420008, Russia

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 2005年 / 43卷 / 11期

关键词：

NMR; H-1; C-13; residual dipolar couplings; spatial structure; lyotropic medium; gamma-L-glutamyl-L-cysteinylglycine; cetylpyridinium chloride;

D O I：

10.1002/mrc.1650

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The approach based on analysis of the residual H-1-C-13 dipolar couplings in molecules partially aligned in a lyotropic liquid crystalline medium was used in the NMR investigation of the reduced glutathione (Glu-Cys-Gly; GSH) structure in a lyotropic medium (cetylpyridinium chloride-n-hexanol). The spatial structure of GSH in solution was established on the basis of the experimental data for observed couplings only. Copyright (c) 2005 John Wiley & Sons, Ltd.

引用

页码：948 / 951

页数：4

共 19 条

[1]

Alba E., 2002, PROG NUCL MAGN RESON, V40, P175

[2]

Anishetty Sharmila, 2002, BMC Struct Biol, V2, P9, DOI 10.1186/1472-6807-2-9

[3] NMR structural characterization of oligo-N-substituted glycine lead compounds from a combinatorial library [J].

Bradley, EK ;

Kerr, JM ;

Richter, LS ;

Figliozzi, GM ;

Goff, DA ;

Zuckermann, RN ;

Spellmeyer, DC ;

Blaney, JM .

MOLECULAR DIVERSITY, 1997, 3 (01) :1-15

[4] Measurement of residual dipolar couplings of macromolecules aligned in the nematic phase of a colloidal suspension of rod-shaped viruses [J].

Clore, GM ;

Starich, MR ;

Gronenborn, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (40) :10571-10572

[5] A novel interactive tool for rigid-body modeling of multi-domain macromolecules using residual dipolar couplings [J].

Dosset, P ;

Hus, JC ;

Marion, D ;

Blackledge, M .

JOURNAL OF BIOMOLECULAR NMR, 2001, 20 (03) :223-231

[6]

Ernst R. R., 1987, PRINCIPLES NUCL MAGN

[7] CONFORMATIONAL STUDY OF GLUTATHIONE BY NMR [J].

FUJIWARA, S ;

FORMICKAKOZLOWSKA, G ;

KOZLOWSKI, H .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1977, 50 (12) :3131-3135

[8]

Jackman L. M., 1975, DYNAMIC NUCL MAGNETI

[9] Spatial structure of triglycine determined by the residual dipolar coupling analysis [J].

Klochkov, VV ;

Khairutdinov, BI ;

Klochkov, AV ;

Shtyrlin, VG ;

Shaykhutdinov, RA .

APPLIED MAGNETIC RESONANCE, 2003, 25 (01) :113-119

[10] NMR measurement of dipolar couplings in proteins aligned by transient binding to purple membrane fragments [J].

Koenig, BW ;

Hu, JS ;

Ottiger, M ;

Bose, S ;

Hendler, RW ;

Bax, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (06) :1385-1386

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