Allele mining of TaGRF-2D gene 5′-UTR in Triticum aestivum and Aegilops tauschii genotypes

被引：9

作者：

Kroupin, Pavel Yu. ^{[1
]}

Chernook, Anastasiya G. ^{[1
]}

Bazhenov, Mikhail S. ^{[1
]}

Karlov, Gennady I. ^{[1
]}

Goncharov, Nikolay P. ^{[2
]}

Chikida, Nadezhda N. ^{[3
]}

Divashuk, Mikhail G. ^{[1
,4
,5
]}

机构：

[1] All Russia Res Inst Agr Biotechnol, Lab Appl Genom & Crop Breeding, Moscow, Russia

[2] Russian Acad Sci, Siberian Branch, Inst Cytol & Genet, Novosibirsk, Russia

[3] All Russian Inst Plant Genet Resources, Fed Res Ctr Vavilov, St Petersburg, Russia

[4] Russian State Agr Univ, Moscow Timiryazev Agr Acad, Ctr Mol Biotechnol, Moscow, Russia

[5] All Russia Res Inst Agr Biotechnol, Kurchatov Genom Ctr ARRIAB, Moscow, Russia

来源：

PLOS ONE | 2020年 / 15卷 / 04期

基金：

俄罗斯科学基金会;

关键词：

BREAD WHEAT; DWARFING GENES; POLYPLOID WHEAT; CHROMOSOME; DIVERSITY; EVOLUTION; IDENTIFICATION; GLAUCOUSNESS; RESISTANCE; RHT-B1B;

D O I：

10.1371/journal.pone.0231704

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The low diversity of the D-subgenome of bread wheat requires the involvement of new alleles for breeding. In grasses, the allelic state of Growth Regulating Factor (GRF) gene is correlated with nitrogen uptake. In this study, we characterized the sequence of TaGRF-2D and assessed its diversity in bread wheat and goatgrass Aegilops tauschii (genome DD). In silico analysis was performed for reference sequence searching, primer pairs design and sequence assembly. The gene sequence was obtained using Illumina and Sanger sequencing. The complete sequences of TaGRF-2D were obtained for 18 varieties of wheat. The polymorphism in the presence/absence of two GCAGCC repeats in 5' UTR was revealed and the GRF-2D-SSR marker was developed. Our results showed that the alleles 5' UTR-250 and 5' UTR-238 were present in wheat varieties, 5' UTR-250 was presented in the majority of wheat varieties. In Ae. tauschii ssp. strangulata (likely donor of the D-subgenome of polyploid wheat), most accessions carried the 5' UTR-250 allele, whilst most Ae. tauschii ssp. tauschii have 5' UTR-244. The developed GRF-2D-SSR marker can be used to study the genetic diversity of wheat and Ae. tauschii.

引用

页数：16

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