Ultrasound Quantification of Molecular Marker Concentration in Large Blood Vessels

被引:4
作者
Wang, Shiying [1 ]
Mauldin, F. William, Jr. [1 ]
Unnikrishnan, Sunil [1 ]
Klibanov, Alexander L. [2 ]
Hossack, John A. [1 ]
机构
[1] Univ Virginia, Dept Biomed Engn, Charlottesville, VA 22908 USA
[2] Univ Virginia, Div Cardiovasc Med, Charlottesville, VA 22908 USA
来源
2014 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IUS) | 2014年
关键词
ultrasound; molecular imaging; quantification; acoustic radiation force; INFLAMMATION;
D O I
10.1109/ULTSYM.2014.0204
中图分类号
O42 [声学];
学科分类号
070206 ; 082403 ;
摘要
Ultrasound-based molecular imaging has been used in pre-clinical studies of cancer and cardiovascular diseases. Current techniques involve the detection of molecularly bound microbubbles through some combination of nonlinear microbubble detection and lengthy waiting periods or low-pass interframe filtering techniques. Non-specific adhesion is typically measured using additional control microbubble injections. In addition to having prolonged protocols, current limitations include the inability to quantify molecular marker concentration in human tissue environments where attenuation and imaging path lengths are highly variable. Consequently, we developed a new modulated Acoustic Radiation Force-based imaging sequence, which can detect targeted adhesion without the requirement of separate control measurements. Results indicate that the metric extracted from the sequence (residual-to-saturation ratio, R-resid) was independent of acoustic pressures and attenuation levels (p > 0.5, n = 10). R-resid exhibited a linear relationship with measured molecular marker concentration (R-2 > 0.94). Consequently, the feasibility was demonstrated in vitro for quantification of molecular marker concentration in large vessel environments. Additionally, the feasibility of using the modulated ARF-based sequence to separate specific adhesion of adherent microbubbles was also demonstrated in a mouse model of inflammation in vivo.
引用
收藏
页码:831 / 834
页数:4
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